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PMID: 109436 Published · ppublish English Journal Article

Arrangement of a highly repeated DNA sequence in the genome and chromatin of the African green monkey.

The Journal of biological chemistry ·Vol. 254 ·No. 12 ·1979-06-25 ·Pages 5506-14

Singer DS

Abstract

The DNA of the African green monkey contains three components that are distinguishable by the kinetics of reassociation. The rapidly reassociating component represents about 20% of the total DNA and is composed almost entirely of a sequence (AGMr(HindIII)-1) which is repeated 6.8 x 10(6) times. The majority of the AGMr(HindIII)-1 sequences are organized in long tandem repeats of a segment of 172 base pairs in length. However, a fraction of the AGMr (HindIII)-1 sequences is interspersed with another 37% of the genome. The structure of the chromatin containing the AGMr-(HindIII)-1 sequence is indistinguishable from that containing total DNA. Furthermore, there is nothing inherent in the nucleotide sequence of AGMr(HindIII)-1 which specifies a unique location for nucleosomes.

MeSH Terms
Animals Base Sequence Cell Line Chlorocebus aethiops Chromatin/analysis DNA Genes Haplorhini Kinetics Nucleic Acid Hybridization Nucleic Acid Renaturation
Chemicals
Chromatin DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Singer D S
Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
1979-06-25
Pages
5506-14
Language
English
Region
United States
NLM ID
2985121R
Subset
IM
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