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PMID: 1096083 Published · ppublish English Journal Article

Number of genes and base composition of mitochondrial tRNA from Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 2 ·No. 6 ·1975-06-00 ·Pages 831-8

Schneller JM, Faye G, Kujawa C, Stahl AJ

Abstract

Increasing amounts of mitochondrial [32P] tRNA (4S fraction), were hybridized with mitochondrial DNA OF Saccharomyces cerevisiae. At saturation, the calculated number of genes for 4S mitochondrial RNA was 20. Mitochondrial [32P] tRNA eluted from the hydrids obtained either with an excess of tRNA or an excess of DNA showed, after alkaline hydrolysis and chromatography, a G+C content of 28 and 35 p. cent respectively. This last value is similar to that found with the total 4S fraction. The odd nucleotides T (about 1T per sequence), U, hU are present in mitochondrial tRNA. Some sequence may begin with pG.

MeSH Terms
Autoradiography DNA, Mitochondrial Electrophoresis, Polyacrylamide Gel Genes Mitochondria/metabolism Nucleic Acid Hybridization RNA, Transfer Ribonucleotides/analysis Saccharomyces cerevisiae/metabolism
Chemicals
DNA, Mitochondrial Ribonucleotides RNA, Transfer
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schneller J M
Faye G
Kujawa C
Stahl A J
References (21)
21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/2.6.831
Published
1975-06-00
Pages
831-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343470
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/2.6.831 PMC Full Text View journal details
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