Production of cellulose microfibrils by Rhizobium.

Applied microbiology ·Vol. 30 ·No. 1 ·1975-07-00 ·Pages 123-31

Napoli C, Dazzo F, Hubbell D

Abstract

Electron microscope examination of Rhizobium spp. revealed microfibrils produced by flocculating strains but not by nonflocculating strains. The microfibrils from R. trifolii (NA30) were isolated and identified as cellulose by enzymatic, X-ray diffraction, and infrared spectral analyses. Both infective and noninfective strains of R. trifolii flocculated and produced microfibrils. More infection threads were observed in clover root hairs growing in the presence of flocs in comparison with root hairs where single bacterial cells predominated.

MeSH Terms

Cell Adhesion Cellulase Cellulose/biosynthesis Microscopy, Electron Microscopy, Phase-Contrast Plant Development Rhizobium/growth & development,metabolism,ultrastructure Spectrophotometry, Infrared Symbiosis X-Ray Diffraction

Chemicals

Cellulose Cellulase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Napoli C

Dazzo F

Hubbell D

References (9)

9 references, click to expand

The role of polymers in microbial aggregation.
Annu Rev Microbiol. 1973;27:27-50 PMID: 4584689
A low-viscosity epoxy resin embedding medium for electron microscopy.
J Ultrastruct Res. 1969 Jan;26(1):31-43 PMID: 4887011
The infection of clover root hairs by nodule bacteria studied by a simple glass slide technique.
J Gen Microbiol. 1957 Apr;16(2):374-81 PMID: 13416514
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
J Cell Biol. 1963 Apr;17:208-12 PMID: 13986422
A convenient ferricyanide estimation of reducing sugars in the nanomole range.
Anal Biochem. 1973 Sep;55(1):321-5 PMID: 4753157
Cellulose: its regeneration in the native lattice.
Science. 1974 Aug 9;185(4150):522-3 PMID: 4841155
Use of infrared analysis in the determination of carbohydrate structure.
Methods Biochem Anal. 1956;3:213-45 PMID: 13369164
Complex forms of cellulase.
Nature. 1958 Nov 1;182(4644):1232-3 PMID: 13590283
Lectins: a possible basis for specificity in the Rhizobium--legume root nodule symbiosis.
Science. 1974 Jul 19;185(4147):269-71 PMID: 17812054

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.30.1.123-131.1975

Published

1975-07-00

Pages

123-31

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC187130

Subset

External Links

PubMed source DOI: 10.1128/am.30.1.123-131.1975 PMC Full Text

Analysis Services

Production of cellulose microfibrils by Rhizobium.

Contact

WeChat Official Account