Liver cirrhosis: immunofluorescence and biochemical studies demonstrate two types of collagen.

Klinische Wochenschrift ·Vol. 53 ·No. 5 ·1975-03-01 ·Pages 205-8

Gay S, Fietzek PP, Remberger K, Eder M, Kühn K

Abstract

Pepsin solubilization of small and large noduled liver cirrhosis yielded two types of collagen (precipitated at 1.7 and 2.5 M NaCl concentrations) as demonstrated by electronmicroscopy. The 1.7 M NaCl precipitate was identified as type III collagen using an immunofluorescence technique. The 2.5 M NaCl precipitate appeared to be type I in the electronmicroscope. However, immunofluorescent and biochemical studies indicated that it was not type I but a type of collagen not yet described.

MeSH Terms

Aged Chemical Precipitation Collagen/analysis,immunology Female Fluorescent Antibody Technique Humans Liver Cirrhosis/immunology,metabolism,pathology Male Microscopy, Electron Middle Aged Molecular Conformation Pepsin A/analysis

Chemicals

Collagen Pepsin A

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Gay S

Fietzek P P

Remberger K

Eder M

Kühn K

References (9)

9 references, click to expand

(Alpha1(3))3 human skin collagen. Release by pepsin digestion and preponderance in fetal life.
J Biol Chem. 1974 May 25;249(10):3225-31 PMID: 4598122
Isolation and characterization of the cyanogen bromide peptides from the alpha 1(3) chain of human collagen.
Biochemistry. 1974 Aug 13;13(17):3459-64 PMID: 4602296
A review of biochemical studies on the genetically distinct collagens of the skeletal system.
Clin Orthop Relat Res. 1973 May;(92):260-80 PMID: 4575876
Cyanogen bromide peptides of type III collagen: first sequence analysis demonstrates homology with type I collagen.
FEBS Lett. 1975 Jan 1;49(3):365-8 PMID: 1109920
The production of specific antibodies to native collagens with the chain compositions, (alpha1(I))3, (alpha1(II))3, and (alpha1(I))2alpha 2.
J Immunol. 1974 Jul;113(1):421-3 PMID: 4832314
Comparative electron-microscope studies on type-III and type-I collagens.
Eur J Biochem. 1975 Feb 21;51(2):363-8 PMID: 1171009
Comparative electron-microscope studies on the collagens extracted from cartilage, bone, and skin.
Eur J Biochem. 1972 May;27(1):192-6 PMID: 4114950
Biochemical properties of human glomerular basement membrane in normal and diabetic kidneys.
J Clin Invest. 1974 Feb;53(2):403-7 PMID: 11344553
Hepatic fibrosis. Correlation of biochemical and morphologic investigations.
Am J Med. 1970 Nov;49:707-21 PMID: 4924592

Article Info

Journal

Klinische Wochenschrift

Abbr.

Klin Wochenschr

ISSN

0023-2173

DOI

10.1007/BF01468808

Published

1975-03-01

Pages

205-8

Language

English

Region

Germany

NLM ID

2985205R

Subset

External Links

PubMed source DOI: 10.1007/BF01468808

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Liver cirrhosis: immunofluorescence and biochemical studies demonstrate two types of collagen.

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