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PMID: 1098043 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Growth of mouse megakaryocyte colonies in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 5 ·1975-05-00 ·Pages 1744-8

Metcalf D, MacDonald HR, Odartchenko N, Sordat B

Abstract

Mouse bone marrow and spleen cells formed pure or mixed colonies of up to 80 megakaryocytes in agar cultures after stimulation by medium conditioned by activated mouse lymphoid cells. Megakaryocytes were identified on the basis of their morphology, polyploid mitoses and DNA content, and high cytoplasmic content of acetylcholinesterase. Megakaryocyte colony-forming cells were relatively small with a peak sedimentation velocity of 4.2 mm/hr. Spleen, lymph node, and thymus cells produced the factor stimulating megakaryocyte proliferation after culture in medium containing 2-mercaptoethanol, with or without added mitogens or allogeneic spleen cells. Peak activity in conditioning medium was associated with the small lymphocyte fractions in mouse spleen.

MeSH Terms
Animals Cell Count Centrifugation Culture Media Culture Techniques/methods DNA/analysis Lymphocytes/cytology Macrophages/analysis Megakaryocytes/analysis,cytology,ultrastructure Mice Mice, Inbred BALB C Microscopy, Phase-Contrast Spleen/cytology Time Factors
Chemicals
Culture Media DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Metcalf D
MacDonald H R
Odartchenko N
Sordat B
References (15)
15 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.5.1744
Published
1975-05-00
Pages
1744-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432622
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.5.1744 PMC Full Text View journal details
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