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PMID: 1098045 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Single-strand scissions of chromosomal DNA during commitment to recombination at meiosis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 5 ·1975-05-00 ·Pages 1887-91

Jacobson GK, Pinon R, Esposito RE, Esposito MS

Abstract

Diploid cells of the yeast Saccharomyces cerevisiae induced to undergo meiosis accumulate single-strand scissions in both template and newly synthesized DNA during commitment to genetic recombination. No evidence for accumulation of double-strand breaks during meiosis was obtained. When commitment to recombination is at the full meiotic level there are approximately 70 to 200 single-strand scissions per meiotic cell in which approximately 150 recombination events have been reported to occur.

MeSH Terms
Centrifugation, Density Gradient Chromosomes/metabolism DNA, Single-Stranded/metabolism Diploidy Meiosis Recombination, Genetic Saccharomyces cerevisiae/metabolism Spores, Fungal/metabolism Templates, Genetic Time Factors Uracil/metabolism
Chemicals
DNA, Single-Stranded Uracil
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jacobson G K
Pinon R
Esposito R E
Esposito M S
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.5.1887
Published
1975-05-00
Pages
1887-91
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432652
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.5.1887 PMC Full Text View journal details
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