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PMID: 10997270 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

A concise guide to cDNA microarray analysis.

BioTechniques ·Vol. 29 ·No. 3 ·2000-09-00 ·Pages 548-50, 552-4, 556 passim

Hegde P, Qi R, Abernathy K, Gay C, Dharap S, Gaspard R, Hughes JE, Snesrud E, Lee N, Quackenbush J

Abstract

Microarray expression analysis has become one of the most widely used functional genomics tools. Efficient application of this technique requires the development of robust and reproducible protocols. We have optimized all aspects of the process, including PCR amplification of target cDNA clones, microarray printing, probe labeling and hybridization, and have developed strategies for data normalization and analysis.

MeSH Terms
DNA, Complementary Humans Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis/methods Polymerase Chain Reaction RNA Probes
Chemicals
DNA, Complementary RNA Probes
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Hegde P
Institute for Genomic Research, Rockville, MD, USA.
Qi R
Abernathy K
Gay C
Dharap S
Gaspard R
Hughes J E
Snesrud E
Lee N
Quackenbush J
Article Info
Journal
BioTechniques
Abbr.
Biotechniques
ISSN
0736-6205
Published
2000-09-00
Pages
548-50, 552-4, 556 passim
Language
English
Region
England
NLM ID
8306785
Subset
IM
Grants
NCI NIH HHS · R01 CA77049-01 · United States
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