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PMID: 11004394 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene.

FEMS microbiology letters ·Vol. 191 ·No. 1 ·2000-10-01 ·Pages 17-24

Roy D, Sirois S

Abstract

The differentiation of Bifidobacterium species was performed with specific primers using the PCR technique, the amplified ribosomal DNA restriction analysis (ARDRA) technique based on reports on the sequence of the 16S rRNA gene and speciation based on a short region of the ldh gene. Four specific primer sets were developed for each of the Bifidobacterium species, B. animalis, B. infantis and B. longum. The use of the ARDRA method made it possible to discriminate between B. infantis, B. longum and B. animalis with the combination of BamHI, TaqI and Sau3AI restriction enzymes. The ldh gene sequences of 309-312 bp were determined for 19 Bifidobacterium strains. Alignment of these short regions of the ldh gene confirmed that it is possible to distinguish between B. longum and B. infantis but not between B. lactis and B. animalis.

MeSH Terms

Amino Acid Sequence Bifidobacterium/classification,genetics DNA Primers DNA Restriction Enzymes DNA, Ribosomal/analysis,genetics Genes, Bacterial Genes, rRNA/genetics L-Lactate Dehydrogenase/genetics Molecular Sequence Data Nucleic Acid Amplification Techniques Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Sequence Alignment Sequence Analysis, DNA Species Specificity

Chemicals

DNA Primers DNA, Ribosomal RNA, Ribosomal, 16S L-Lactate Dehydrogenase DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Roy D

Food Research and Development Centre, Agriculture and Agri-Food Canada, 3600 Casavant Boulevard West, J2S 8E3, St. Hyacinthe, Que., Canada. [email protected]

Sirois S

Article Info

Journal

FEMS microbiology letters

Abbr.

FEMS Microbiol Lett

ISSN

0378-1097

DOI

10.1111/j.1574-6968.2000.tb09313.x

[email protected]

Published

2000-10-01

Pages

17-24

Language

English

Region

England

NLM ID

7705721

Subset

Databases

GENBANK

AF261669, AF261670, AF261671, AF261672, AF261673, AF261674, AF261675, AF261676, AF261677, AF261678, AF261679, AF261680, AF261681, AF261682, AF261683, AF261684, AF261685, AF261686, AF261687

External Links

PubMed source DOI: 10.1111/j.1574-6968.2000.tb09313.x View journal details

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Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene.

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