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PMID: 1100762 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The Fc receptor on thymus-derived lymphocytes. I. Detection of a subpopulation of murine T lymphocytes bearing the Fc receptor.

The Journal of experimental medicine ·Vol. 142 ·No. 3 ·1975-09-01 ·Pages 611-21

Stout RD, Herzenberg LA

Abstract

Utilizing the fluorescence-activated cell sorter (FACS) and washed murine antibody-antigen complexes formed in antibody excess, we have demonstrated the presence of the Fc receptor on the surface of a distinct subpopulation of murine T lymphocytes. No differences in intensity of labeling with the complexes was observed when the Fc+ T lymphocytes were compared with Fc+ B lymphocytes. The majority of Fc+ T lymphocytes are small lymphocytes determined by light-scattering characteristics on the FACS. Separating Fc+ from Fc- T lymphocytes from spleens of mice primed 1 wk or 1 mo previously with keyhole limpet hemocyanin (KLH) revealed that the T cells capable of cooperating with DNP-KLH primed B cells to give an adoptive anti-DNP PFC response do not bear the Fc receptor.

MeSH Terms
Animals Antibodies Antibodies, Anti-Idiotypic Binding Sites, Antibody Cell Membrane/immunology Cell Separation Female Fluorescent Antibody Technique Immunoglobulin Fc Fragments Lymph Nodes/transplantation Male Mice Mice, Inbred BALB C Spleen/transplantation T-Lymphocytes/immunology Thymus Gland/transplantation Transplantation, Homologous Viral Plaque Assay
Chemicals
Antibodies Antibodies, Anti-Idiotypic Immunoglobulin Fc Fragments
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stout R D
Herzenberg L A
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24 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.142.3.611
Published
1975-09-01
Pages
611-21
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189922
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.142.3.611 PMC Full Text View journal details
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