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PMID: 1102942 Published · ppublish English Journal Article

Replication of phiA and phiX174 in Escherichia coli mutants thermosensitive in DNA synthesis.

Molecular & general genetics : MGG ·Vol. 139 ·No. 4 ·1975-09-08 ·Pages 285-91

Taketo A

Abstract

Thermosensitive mutants of E. coli defective in DNA replication were tested for their capacity to support multiplication of phiA and phiX174. At the restrictive temperature, the viral growth was markedly affected in dnaH, dnaZ, or ligts7 mutants. Even when these strains were transfected with RF1 molecules, the virus yield was still very low. The dnaI function was, however, dispensable for replication of phiA and phiX174. In addition, these viruses could multiply in dnaP or polAts mutants at the high temperature.

MeSH Terms
Coliphages/growth & development DNA Nucleotidyltransferases DNA Replication DNA, Viral/analysis,metabolism Escherichia coli/metabolism Glycine Hot Temperature Lysogeny Mutation Polynucleotide Ligases/metabolism Spheroplasts/growth & development Virus Replication
Chemicals
DNA, Viral DNA Nucleotidyltransferases Polynucleotide Ligases Glycine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Taketo A
References (20)
20 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267968
Published
1975-09-08
Pages
285-91
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267968
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