Home LiteratureArticle Details

PMID: 11030352 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

E2F4 and E2F5 play an essential role in pocket protein-mediated G1 control.

Molecular cell ·Vol. 6 ·No. 3 ·2000-09-00 ·Pages 729-35

Gaubatz S, Lindeman GJ, Ishida S, Jakoi L, Nevins JR, Livingston DM, Rempel RE

Abstract

E2F transcription factors are major regulators of cell proliferation. The diversity of the E2F family suggests that individual members perform distinct functions in cell cycle control. E2F4 and E2F5 constitute a defined subset of the family. Until now, there has been little understanding of their individual biochemical and biological functions. Here, we report that simultaneous inactivation of E2F4 and E2F5 in mice results in neonatal lethality, suggesting that they perform overlapping functions during mouse development. Embryonic fibroblasts isolated from these mice proliferated normally and reentered from Go with normal kinetics compared to wild-type cells. However, they failed to arrest in G1 in response to p16INK4a. Thus, E2F4 and E2F5 are dispensable for cell cycle progression but necessary for pocket protein-mediated G1 arrest of cycling cells.

MeSH Terms

Alleles Animals Carrier Proteins/genetics Cell Survival/genetics Cyclin-Dependent Kinase Inhibitor p16 Cyclin-Dependent Kinase Inhibitor p21 Cyclins/genetics DNA-Binding Proteins/genetics,metabolism E2F4 Transcription Factor E2F5 Transcription Factor Fibroblasts/cytology G1 Phase/physiology Gene Expression Regulation, Developmental Genes, ras/physiology Genotype Heterozygote Homozygote Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Nuclear Proteins/genetics,metabolism Phenotype Phosphoproteins/genetics,metabolism Phosphorylation Proteins Retinoblastoma Protein/genetics,metabolism Retinoblastoma-Like Protein p107 Retinoblastoma-Like Protein p130 Transcription Factors/genetics,metabolism Transcription, Genetic/physiology Transfection

Chemicals

Carrier Proteins Cdkn1a protein, mouse Cyclin-Dependent Kinase Inhibitor p16 Cyclin-Dependent Kinase Inhibitor p21 Cyclins DNA-Binding Proteins E2F4 Transcription Factor E2F5 Transcription Factor E2f5 protein, mouse Nuclear Proteins Phosphoproteins Proteins Rbl1 protein, mouse Rbl2 protein, mouse Retinoblastoma Protein Retinoblastoma-Like Protein p107 Retinoblastoma-Like Protein p130 Transcription Factors

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Gaubatz S

The Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA.

Lindeman G J

Ishida S

Jakoi L

Nevins J R

Livingston D M

Rempel R E

Article Info

Journal

Molecular cell

Abbr.

Mol Cell

ISSN

1097-2765

DOI

10.1016/s1097-2765(00)00071-x

Published

2000-09-00

Pages

729-35

Language

English

Region

United States

NLM ID

9802571

Subset

External Links

PubMed source DOI: 10.1016/s1097-2765(00)00071-x View journal details

Analysis Services

E2F4 and E2F5 play an essential role in pocket protein-mediated G1 control.

Contact

WeChat Official Account