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PMID: 11041877 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Separable whorl-specific expression and negative regulation by enhancer elements within the AGAMOUS second intron.

The Plant cell ·Vol. 12 ·No. 10 ·2000-10-00 ·Pages 1799-810

Deyholos MK, Sieburth LE

Abstract

We analyzed the 4-kb intragenic control region of the AGAMOUS (AG) gene to gain insight into the mechanisms controlling its expression during early flower development. We identified three major expression patterns conferred by 19 AG::reporter gene constructs: the normal AG pattern, a stamen-specific pattern, and a predominantly carpel pattern. To determine whether these three expression patterns were under negative control by APETALA2 (AP2) or LEUNIG (LUG), we analyzed beta-glucuronidase staining patterns in Arabidopsis plants homozygous for strong ap2 and lug mutations. Our results indicated that the stamen-specific pattern was independent of AP2 but dependent on LUG; conversely, the carpel-specific pattern was independent of LUG but dependent on AP2. These results lead to a model of control of AG expression such that expression in each of the two inner whorls is under independent positive and negative control.

MeSH Terms
AGAMOUS Protein, Arabidopsis Arabidopsis/genetics Arabidopsis Proteins DNA-Binding Proteins/genetics Enhancer Elements, Genetic Gene Expression Regulation, Plant Glucuronidase/genetics Homeodomain Proteins/metabolism Homozygote Introns Molecular Sequence Data Nuclear Proteins/metabolism Plant Proteins/genetics,metabolism Plant Stems/metabolism Plants, Genetically Modified
Chemicals
AGAMOUS Protein, Arabidopsis APETALA2 protein, Arabidopsis Arabidopsis Proteins DNA-Binding Proteins Homeodomain Proteins Nuclear Proteins Plant Proteins Glucuronidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deyholos M K
Biology Department, McGill University, Montreal, Quebec, Canada H3A 1B1.
Sieburth L E
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.12.10.1799
Published
2000-10-00
Pages
1799-810
Language
English
Region
England
NLM ID
9208688
PMCID
PMC149120
Subset
IM
Databases
GENBANK
AL021711
External Links
PubMed source DOI: 10.1105/tpc.12.10.1799 PMC Full Text View journal details
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