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PMID: 11050187 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

"Kiss and run" exocytosis at hippocampal synapses.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 23 ·2000-11-07 ·Pages 12828-33

Stevens CF, Williams JH

Abstract

We have combined electrophysiology and imaging to measure the release of neurotransmitter and fluorescent dye at synapses of cultured hippocampal neurons. These experiments have revealed a "kiss and run" mode of exocytosis in which synaptic vesicles release glutamate normally but do not permit dye to enter or escape from the vesicle. During "kiss and run," the vesicle interior may be exposed very transiently (<6 ms), or a special configuration of the fusion pore may prevent dye exchange. We estimate that about 20% of the vesicles normally use this "kiss and run" pathway, and that the fraction of "kiss and run" events can be increased to over 80% by superfusing the synapses with hypertonic solution.

MeSH Terms
Action Potentials Animals Calcium/metabolism Cells, Cultured Coloring Agents/metabolism Electrophysiology Exocytosis/physiology Glutamates/metabolism Hippocampus/cytology,physiology Neurons/cytology,physiology Neurotransmitter Agents/metabolism Osmolar Concentration Presynaptic Terminals/physiology Rats Synapses/physiology Synaptic Transmission/physiology Synaptic Vesicles/metabolism
Chemicals
Coloring Agents Glutamates Neurotransmitter Agents Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stevens C F
Howard Hughes Medical Institute, and Molecular Neurobiology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA. [email protected]
Williams J H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.230438697
Email
[email protected]
Published
2000-11-07
Pages
12828-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC18849
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.230438697 PMC Full Text View journal details
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