panD, a new chromosomal locus of Salmonella typhimurium for the biosynthesis of beta-alanine.

Molecular & general genetics : MGG ·Vol. 140 ·No. 2 ·1975-09-29 ·Pages 159-64

Ortega MV, Cárdenas A, Ubiera D

Abstract

Three mutants of Salmonella typhimurium LT2, which required either pantothenate or beta-alanine for growth, were obtained after treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Their phenotype was: SM30 Pan-, SM31 Pan- Met-, SM32 Pan- Thi- (requirement for the thiazole-moiety of thiamine). Neither aspartate, dihydrouracil, nor beta-ureidopropionate replaced beta-alanine as growth factor. By conjugation it was found that the three genetic lesions (Pan-, Met-, Thi-) were located at about minute 128 of the bacterial chromosome. By transduction 63% linkage was found between the Pan and Met loci, and 84% between the Thi and Pan loci. Probably the thiazole auxotrophy was due to a lesion in the thiG locus. The new genetic locus responsible for the synthesis of beta-alanine was named panD.

MeSH Terms

Alanine/biosynthesis Chromosome Mapping Chromosomes, Bacterial Genetic Linkage Mutation Pantothenic Acid/metabolism Phenotype Salmonella typhimurium/metabolism

Chemicals

Pantothenic Acid Alanine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ortega M V

Cárdenas A

Ubiera D

References (15)

15 references, click to expand

Mutation of Salmonella typhimurium by nitrosoguanidine.
Mutat Res. 1965 Feb;2(1):1-10 PMID: 5341286
STUDIES ON THE ASPARTIC ACID DECARBOXYLASE OF RHIZOBIUM TRIFOLII.
J Bacteriol. 1949 Aug;58(2):215-21 PMID: 16561774
Mutants of Escherichia coli requiring methionine or vitamin B12.
J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
Dihydrouracil as a growth factor for a mutant strain of Escherichia coli.
J Bacteriol. 1956 Aug;72(2):276-7 PMID: 13366911
Growth inhibition by hexoses of a temperature-sensitive thiazoleless mutant of Salmonella typhimurium.
J Bacteriol. 1967 Sep;94(3):707-11 PMID: 5340682
Metabolism of intermediate pyrimidine reduction products in vitro.
J Biol Chem. 1956 Jan;218(1):1-7 PMID: 13278308
Pantothenate studies. I. Interference by D-serine and L-aspartic acid with pantothenate synthesis in Escherichia coli.
J Bacteriol. 1950 Dec;60(6):733-45 PMID: 14824065
On the process of cellular division in Escherichia coli. I. Asymmetrical cell division and production of deoxyribonucleic acid-less bacteria.
J Mol Biol. 1968 Jul 14;35(1):175-92 PMID: 4939776
Dihydrouracil as a growth factor for mutant strains of Escherichia coli.
J Bacteriol. 1957 Aug;74(2):122-5 PMID: 13475206
THE LINKAGE MAP OF SALMONELLA TYPHIMURIUM.
Genetics. 1965 Jun;51:897-913 PMID: 14337763
A proposal for a uniform nomenclature in bacterial genetics.
Genetics. 1966 Jul;54(1):61-76 PMID: 5961488
Mutagenesis of the replication point by nitrosoguanidine: map and pattern of replication of the Escherichia coli chromosome.
J Mol Biol. 1968 May 14;33(3):705-19 PMID: 4882616
Linkage map of Salmonella typhimurium, edition IV.
Bacteriol Rev. 1972 Dec;36(4):558-86 PMID: 4568764
Pantothenate studies. III. Description of the extracted pantothenate-synthesizing enzyme of Escherichia coli.
J Biol Chem. 1952 Sep;198(1):23-32 PMID: 12999714
F + , Hfr, and F' strains of Salmonella typhimurium and Salmonella abony.
Bacteriol Rev. 1972 Dec;36(4):608-37 PMID: 4568766

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00329783

Published

1975-09-29

Pages

159-64

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00329783

Analysis Services

panD, a new chromosomal locus of Salmonella typhimurium for the biosynthesis of beta-alanine.

Contact

WeChat Official Account