Binding of the complement intermediate C56 to zymosan in acute phase human sera.
C56 is known to appear in the fluid phase when zymosan is incubated at 37 degrees C with certain acute phase 'reactor' sera. In the present study, C56 was detected bound to the zymosan particle prior to its appearance free in solution. In reactor sera C56 was formed and released with kinetics similar to that of the generation and decay of a C56-binding site formed when zymosan was incubated with normal serum. Bound and fluid phase C56 was detected only in reactor sera, and was generated only by agents known preferentially to activate the properdin pathway. Elution of C56 from zymosan in hypertonic salt solutions proved to be a convenient step in the partial purification of large amounts of this haemolytically active bimolecular complex.
15 references, click to expand
-
Studies of complement complex C5b,6 eluted from--EAC-6: reaction of C5b,6 with EAC4b,3b and evidence on the role of C2a and C3b in the activation of C5.J Immunol. 1974 Sep;113(3):998-1007 PMID: 4213402
-
Reactive lysis: the complement-mediated lysis of unsensitized cells. I. The characterization of the indicator factor and its identification as C7.J Exp Med. 1970 Apr 1;131(4):629-41 PMID: 4193934
-
Reaction mechanisms of nascent C567 (reactive lysis). I. Reaction characteristics for production of EC567 and lysis by C8 and C9.J Immunol. 1972 Aug;109(2):353-9 PMID: 4558539
-
Studies on the inhibition of C56-initiated lysis (reactive lysis). II. C567-INH--an inhibitor of the C567 trimolecular complex of complement.Int Arch Allergy Appl Immunol. 1974;47(4):623-32 PMID: 4415956
-
Kinetic studies of the formation of the properdin system enzymes on zymosan: evidence that nascent C3b controls the rate of assembly.J Immunol. 1974 Mar;112(3):1115-23 PMID: 4811964
-
The reaction of zymosan with the properdin system in normal and C4-deficienct guinea pig serum. Demonstration of C3- and C5-cleaving multi-unit enzymes, both containing factor B, and acceleration of their formation by the classical complement pathway.J Immunol. 1973 Nov;111(5):1389-400 PMID: 4795621
-
Molecular analysis of the membrane attack mechanism of complement.J Exp Med. 1972 Mar 1;135(3):549-66 PMID: 5058233
-
Studies on the inhibition of C56-initiated lysis (reactive lysis). III. Characterization of the inhibitory activity C567-INH and its mode of action.Immunology. 1975 Jan;28(1):133-49 PMID: 1116868
-
The fifth component of the guinea pig complement system. 3. Dissociation and transfer of C5b, and the probable site of C5b fixation.J Immunol. 1971 Feb;106(2):480-93 PMID: 5545154
-
Lysis of erythrocytes by complement in the absence of antibody.J Exp Med. 1970 Nov;132(5):898-915 PMID: 5470509
-
Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum.Immunochemistry. 1966 Mar;3(2):111-35 PMID: 5960883
-
Studies on the inhibition of C56 initiated lysis (reactive lysis). I. Description of the phenomenon and methods of assay.Immunology. 1974 Jun;26(6):1145-57 PMID: 4137213
-
Reactive haemolysis--a distinctive form of red cell lysis.Immunology. 1968 May;14(5):745-62 PMID: 4172980
-
Reactive lysis: the complement-mediated lysis of unsensitized cells. II. The characterization of activated reactor as C56 and the participation of C8 and C9.J Exp Med. 1970 Apr 1;131(4):643-57 PMID: 4193935
-
Reaction mechanisms of nascent C567 (reactive lysis). II. Killing of a rough form of Escherichia coli by C567, C8, and C9.J Infect Dis. 1974 Apr;129(4):444-50 PMID: 4206245
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
