Home LiteratureArticle Details

PMID: 11071873 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Frequent altered expression of fragile histidine triad protein in human colorectal adenomas.

Biochemical and biophysical research communications ·Vol. 278 ·No. 1 ·2000-11-11 ·Pages 205-10

Morikawa H, Nakagawa Y, Hashimoto K, Niki M, Egashira Y, Hirata I, Katsu K, Akao Y

Abstract

Fragile histidine triad (FHIT) gene is involved in deletions on the short arm of chromosome 3 in various human cancers. We found that 47% of colorectal adenomas, which is a higher frequency than that of K-ras, showed altered expression of the Fhit protein by Western blot analysis. The amount of Fhit protein was inversely correlated with the degree of dysplasia. Importantly, 27% of low-grade dysplastic adenomas showed altered expression of Fhit protein. Additionally, expression of human Fhit protein in human colon carcinoma cell line SW480 exhibited a marked inhibition of growth and rendered SW480 cells highly susceptible to undergo apoptosis compared with control cells. These findings suggest that altered expression of the FHIT gene is a quite early aberration in the development of colorectal tumors and that Fhit protein may act as a tumor suppressor.

MeSH Terms

Acid Anhydride Hydrolases Adenoma/metabolism Adult Aged Apoptosis Blotting, Western Cell Division Chromosomes, Human, Pair 3 Cloning, Molecular Colon/metabolism,pathology Colorectal Neoplasms/metabolism DNA Fragmentation Female Gene Deletion Humans Hydrogen Peroxide/pharmacology Hydrolases Immunohistochemistry Male Middle Aged Mucous Membrane/metabolism Neoplasm Proteins Nucleosomes/metabolism Polymorphism, Single-Stranded Conformational Protein Biosynthesis Proto-Oncogene Proteins p21(ras)/biosynthesis Rectum/metabolism,pathology Reverse Transcriptase Polymerase Chain Reaction Transfection Tumor Cells, Cultured

Chemicals

Neoplasm Proteins Nucleosomes fragile histidine triad protein histidine triad protein Hydrogen Peroxide Hydrolases Acid Anhydride Hydrolases HRAS protein, human Proto-Oncogene Proteins p21(ras)

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Morikawa H

Second Department of Internal Medicine, Osaka Medical College, Daigaku-cho, Takatsuki, Osaka 569-8686, Japan.

Nakagawa Y

Hashimoto K

Niki M

Egashira Y

Hirata I

Katsu K

Akao Y

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1006/bbrc.2000.3771

Published

2000-11-11

Pages

205-10

Language

English

Region

United States

NLM ID

0372516

Subset

External Links

PubMed source DOI: 10.1006/bbrc.2000.3771 View journal details

Analysis Services

Frequent altered expression of fragile histidine triad protein in human colorectal adenomas.

Contact

WeChat Official Account