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PMID: 1107307 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

cis-Dominant, transfer-deficient mutants of the Escherichia coli K-12 F sex factor.

Journal of bacteriology ·Vol. 125 ·No. 1 ·1976-01-00 ·Pages 233-47

Guyer MS, Clark AJ

Abstract

Rare conjugational progeny formed by crossing each of five Hfr strains with a recA-F- strain have been characterized. Selection was made for a proximal Hfr marker, taking strict precautions to prevent transfer of recA+ to the zygotes. Most of the progeny were found to be F' strains containing deletion mutant plasmids. With two exceptions, these mutant plasmids have lost all of the tra genes, which are required to confer conjugational donor ability upon a host. In addition, all but the exceptional mutant plasmids were found to be very poorly transmissible from transient heterozygotes which also contain a wild-type F' plasmid. The poor transmissibility is a cis-dominant transfer-defective phenotype which may result from deletion of all or part of the origin of transfer replication (ori), or of a gene determining a cis-acting protein. The two exceptional mutant plasmids may carry short deletions of some of the tra genes or polar tra mutations. The remaining progeny were nonmutant F' strains and F- strains. The frequency with which the F- strains were recovered permits us to estimate that the maximum amount of recombination possible in a recA56 zygote is 10(-6) that of a recA+ zygote.

MeSH Terms
Conjugation, Genetic DNA, Bacterial/analysis DNA, Circular/analysis Escherichia coli/analysis F Factor Genes, Dominant Heterozygote Mutation Recombination, Genetic
Chemicals
DNA, Bacterial DNA, Circular
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guyer M S
Clark A J
References (43)
43 references, click to expand
  1. GROWTH OF MALE-SPECIFIC BACTERIOPHAGE IN PROTEUS MIRABILIS HARBORING F-GENOTES DERIVED FROM ESCHERICHIA COLI.
    J Bacteriol. 1965 May;89:1231-6 PMID: 14292991
  2. Circular DNA.
    Annu Rev Biochem. 1971;40:899-942 PMID: 4942224
  3. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12.
    Proc Natl Acad Sci U S A. 1965 Feb;53:451-9 PMID: 14294081
  4. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli.
    J Mol Biol. 1962 Dec;5:618-34 PMID: 13970180
  5. Transduction of the attached sex factor of Escherichia coli.
    J Bacteriol. 1962 Mar;83:673-8 PMID: 13885924
  6. [System of repression insuring immunity in lysogenic bacteria].
    C R Hebd Seances Acad Sci. 1959 Jun 1;248(22):3219-21 PMID: 13663329
  7. Linkage map of Escherichia coli strain K-12.
    Bacteriol Rev. 1972 Dec;36(4):504-24 PMID: 4568762
  8. A proposal for a uniform nomenclature in bacterial genetics.
    Genetics. 1966 Jul;54(1):61-76 PMID: 5961488
  9. Extrachromosomal inheritance in bacteria.
    Bacteriol Rev. 1969 Jun;33(2):210-63 PMID: 4896350
  10. T7 translational control mechanisms and their inhibiton by F factors.
    Nat New Biol. 1971 May 12;231(19):37-41 PMID: 5283385
  11. Transfer-defective mutants of sex factors in Escherichia coli. II. Deletion mutants of an F-prime and deletion mapping of cistrons involved in genetic transfer.
    Genetics. 1970 Feb;64(2):189-97 PMID: 4919135
  12. Transfer-defective mutants of sex factors in Escherichia coli. I. Defective mutants and complementation analysis.
    Genetics. 1970 Feb;64(2):173-88 PMID: 4919134
  13. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.
    Biochemistry. 1970 Oct 27;9(22):4428-40 PMID: 4919547
  14. Production of a merodiploid strain from a double male strain of E. coli K12.
    Mol Gen Genet. 1969;105(1):1-15 PMID: 4899397
  15. Genetic analysis of recombination-deficient mutants of Escherichia coli K-12 carrying rec mutations cotransducible with thyA.
    J Bacteriol. 1969 Nov;100(2):923-34 PMID: 4901366
  16. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants.
    J Bacteriol. 1971 May;106(2):529-38 PMID: 4929865
  17. Isolation of transducing particles of phi-80 bacteriophage that carry different regions of the Escherichia coli genome.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):795-8 PMID: 4927673
  18. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12.
    Proc Natl Acad Sci U S A. 1968 May;60(1):160-7 PMID: 4873517
  19. Effects of temperature, agitation, and donor strain on chromosome transfer in Escherichia coli K-12.
    J Bacteriol. 1968 Dec;96(6):2077-84 PMID: 4881703
  20. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.
    J Bacteriol. 1969 Jan;97(1):244-9 PMID: 4884815
  21. Permeability lesions in male Escherichia coli infected with bacteriophage T7.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2222-6 PMID: 1094459
  22. A link between streptomycin and rifampicin mutation.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2084-7 PMID: 1094452
  23. Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor.
    Mol Gen Genet. 1971;111(3):256-64 PMID: 5563936
  24. The genetics of transmissible plasmids.
    Annu Rev Genet. 1972;6:257-68 PMID: 4352831
  25. Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus.
    J Mol Biol. 1972 Jul 21;68(2):285-302 PMID: 4262657
  26. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. IX. Note on the deletion mutant of F, F delta(33-43).
    J Mol Biol. 1974 Nov 15;89(4):647-50 PMID: 4615164
  27. Strand and site specificity of the relaxation event for the relaxation complex of the antibiotic resistance plasmid R6K.
    Biochemistry. 1974 Dec 31;13(27):5484-90 PMID: 4616719
  28. The site of action of the F transfer inhibitor.
    Mol Gen Genet. 1973 Dec 31;127(4):307-16 PMID: 4594003
  29. F factor-mediated immunity to lethal zygosis in Escherichia coli K-12.
    J Bacteriol. 1974 Jan;117(1):100-6 PMID: 4587600
  30. Location of the origin of transfer of the sex factor F.
    J Bacteriol. 1972 Nov;112(2):773-8 PMID: 4565418
  31. Characterization of the F transfer cistron, traL.
    Genet Res. 1973 Apr;21(2):205-13 PMID: 4574420
  32. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  33. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.
    J Mol Biol. 1972 Nov 14;71(2):471-97 PMID: 4564488
  34. Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1549-53 PMID: 4598307
  35. Mapping loci for surface exclusion and incompatibility on the F factor of Escherichia coli K-12.
    J Bacteriol. 1974 Jun;118(3):778-82 PMID: 4598002
  36. Cis-limited action of the gene-A product of bacteriophage phiX174 and the essential bacterial site (E. coli-electron microscopy-cis-acting protein-specifically-nicked RF).
    Proc Natl Acad Sci U S A. 1972 Feb;69(2):475-9 PMID: 4551145
  37. Early transfer of genes determining transfer functions by some Hfr strains in Escherichia coli K12.
    Mol Gen Genet. 1972;116(4):336-47 PMID: 4560589
  38. Genetic and physicochemical characterization of Escherichia coli strains carrying fused F' elements derived from KLF1 and F57.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1481-5 PMID: 4556462
  39. Deletion map of the Escherichia coli K-12 sex factor F: the order of eleven transfer cistrons.
    J Bacteriol. 1972 Jun;110(3):857-63 PMID: 4555414
  40. Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation.
    J Bacteriol. 1972 Jun;110(3):843-51 PMID: 4555413
  41. Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli.
    J Bacteriol. 1972 Jun;110(3):831-42 PMID: 4555412
  42. Phage T7 lysozyme mRNA transcription and translation in vivo and in vitro.
    Biochem Biophys Res Commun. 1971 Oct 15;45(2):315-20 PMID: 4946267
  43. A QUICK AND EFFICIENT METHOD FOR INTERRUPTION OF BACTERIAL CONJUGATION.
    Genet Res. 1965 Jul;6:300-3 PMID: 14345913
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.125.1.233-247.1976
Published
1976-01-00
Pages
233-47
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233357
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.125.1.233-247.1976 PMC Full Text View journal details
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