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PMID: 11075367 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes.

Methods in enzymology ·Vol. 328 ·2000-00-00 ·Pages 575-92

Walhout AJ, Temple GF, Brasch MA, Hartley JL, Lorson MA, van den Heuvel S, Vidal M

Abstract

暂无摘要

MeSH Terms

Animals Base Sequence Caenorhabditis/genetics Cloning, Molecular/methods DNA Primers Escherichia coli/genetics Genetic Vectors Genome Helicobacter pylori/genetics Humans Open Reading Frames Polymerase Chain Reaction/methods Recombination, Genetic Saccharomyces cerevisiae

Chemicals

DNA Primers

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Walhout A J

Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.

Temple G F

Brasch M A

Hartley J L

Lorson M A

van den Heuvel S

Vidal M

Article Info

Journal

Methods in enzymology

Abbr.

Methods Enzymol

ISSN

0076-6879

DOI

10.1016/s0076-6879(00)28419-x

Published

2000-00-00

Pages

575-92

Language

English

Region

United States

NLM ID

0212271

Subset

Grants

NCI NIH HHS · 1 R21 CA81658A01 · United States

NHGRI NIH HHS · 1 RO1 HG01715-01 · United States

External Links

PubMed source DOI: 10.1016/s0076-6879(00)28419-x View journal details

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GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes.

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