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PMID: 1108020 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning of DNA from F sex factor of Escherichia coli K-12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 1 ·1976-01-00 ·Pages 64-8

Skurray RA, Nagaishi H, Clark AJ

Abstract

We describe the molecular cloning of various DNA segments generated by partial EcoRI endonuclease digestion of the sex factor F. These segments have been analyzed by agarose gel electrophoresis of EcoRI digests and were arranged in a series of overlapping fragments using the EcoRI fragment map of F established by H. Ohtsubo and E. Ohtsubo. The clones isolated demonstrate one or more of the following F-specified functions: inhibition of female-specific phage (T7) multiplication, formation of F pili, surface exclusion, or immunity to lethal zygosis. These properties are discussed in terms of the EcoRI fragments of F that specify them.

MeSH Terms
Coliphages/metabolism Conjugation, Genetic DNA Restriction Enzymes DNA, Bacterial/analysis,biosynthesis,metabolism Escherichia coli/ultrastructure Extrachromosomal Inheritance F Factor Genotype Operon Phenotype Plasmids
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Skurray R A
Nagaishi H
Clark A J
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.1.64
Published
1976-01-00
Pages
64-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC335839
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.1.64 PMC Full Text View journal details
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