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PMID: 11083916 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Doublecortin-like kinase is associated with microtubules in neuronal growth cones.

Molecular and cellular neurosciences ·Vol. 16 ·No. 5 ·2000-11-00 ·Pages 529-41

Burgess HA, Reiner O

Abstract

Doublecortin-like kinase (DCLK) shares sequence similarity to Doublecortin (DCX) in its N-terminal region. It contains the evolutionary conserved DC repeat motif as well a C-terminal kinase domain. Ectopic expression of DCLK in COS cells results in colocalization with microtubules, and phosphorylated DCLK copurifies with microtubules during assembly from embryonic brain extract. During brain development DCLK is expressed mainly in postmigratory neurons in a similar pattern to DCX. We demonstrate that DCLK is a microtubule-associated active protein kinase expressed in growth cones of postmitotic neurons.

MeSH Terms
Animals COS Cells Calcium-Calmodulin-Dependent Protein Kinases/analysis,genetics,metabolism Cell Movement/physiology Cerebral Cortex/abnormalities,cytology,embryology Doublecortin Domain Proteins Gene Expression Regulation, Developmental Genes, Reporter Green Fluorescent Proteins Growth Cones/chemistry,metabolism In Situ Hybridization Indicators and Reagents/metabolism Luminescent Proteins/genetics Microtubule-Associated Proteins Microtubules/metabolism Mitosis Nerve Tissue Proteins Neurons/physiology,ultrastructure Neuropeptides/genetics,metabolism RNA, Messenger/analysis Transfection
Chemicals
Doublecortin Domain Proteins Indicators and Reagents Luminescent Proteins Microtubule-Associated Proteins Nerve Tissue Proteins Neuropeptides RNA, Messenger Green Fluorescent Proteins Calcium-Calmodulin-Dependent Protein Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Burgess H A
Department of Molecular Genetics, The Weizmann Institute of Science, 76100 Rehovot, Israel.
Reiner O
Article Info
Journal
Molecular and cellular neurosciences
Abbr.
Mol Cell Neurosci
ISSN
1044-7431
DOI
10.1006/mcne.2000.0891
Published
2000-11-00
Pages
529-41
Language
English
Region
United States
NLM ID
9100095
Subset
IM
External Links
PubMed source DOI: 10.1006/mcne.2000.0891 View journal details
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