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PMID: 1108792 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Improved method of selection for mutants of Pseudomonas putida.

Applied microbiology ·Vol. 30 ·No. 6 ·1975-12-00 ·Pages 1046-7

Carhart G, Hegeman G

Abstract

Optimum conditions for enrichment of mutants of Pseudomonas putida in liquid culture were established using a procedure which combines N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis with an improved D-cycloserine selection.

MeSH Terms

Bacteriological Techniques Culture Media Cycloserine Histidine/metabolism Methylnitronitrosoguanidine Mutagens Mutation Pseudomonas/isolation & purification,metabolism

Chemicals

Culture Media Mutagens Methylnitronitrosoguanidine Histidine Cycloserine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Carhart G

Hegeman G

References (4)

4 references, click to expand

Hybridization between Escherichia coli and Shigella.
J Bacteriol. 1957 Oct;74(4):461-76 PMID: 13475269
Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type.
J Bacteriol. 1966 Mar;91(3):1140-54 PMID: 5929747
Isolation of spontaneous mutant strains of Pseudomonas putida.
Biochem Biophys Res Commun. 1969 Jul 7;36(1):179-84 PMID: 5796751
Kinetic studies of pigment synthesis by non-sulfur purple bacteria.
J Cell Physiol. 1957 Feb;49(1):25-68 PMID: 13416343

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.30.6.1046-1047.1975

Published

1975-12-00

Pages

1046-7

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC376589

Subset

External Links

PubMed source DOI: 10.1128/am.30.6.1046-1047.1975 PMC Full Text

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Improved method of selection for mutants of Pseudomonas putida.

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