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PMID: 11090209 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

REP1, a basic helix-loop-helix protein, is required for a branch pathway of phytochrome A signaling in arabidopsis.

The Plant cell ·Vol. 12 ·No. 11 ·2000-11-00 ·Pages 2061-74

Soh MS, Kim YM, Han SJ, Song PS

Abstract

Phytochromes are primary photoreceptors mediating diverse responses ranging from induction of germination to floral induction in higher plants. We have isolated novel recessive rep1 (reduced phytochrome signaling 1) mutants, which exhibit a long-hypocotyl phenotype only under far-red light but not under red light. Physiological characterization showed that rep1 mutations greatly reduced a subset of phytochrome A-regulated responses, including the inhibition of hypocotyl elongation, cotyledon expansion, modulation of gravitropic growth of hypocotyl, and induction of the CAB (encoding chlorophyll a/b binding protein) gene, without affecting the accumulation of anthocyanin, far-red-preconditioned blocking of greening, induction of germination, and induction of CHS (encoding chalcone synthase) and FNR (encoding ferredoxin-NADP(+) oxidoreductase) genes. These results suggest that REP1 is a positive signaling component, functioning in a branch of the phytochrome A signaling pathway. Molecular cloning and characterization of the REP1 gene revealed that it encodes a light-inducible, putative transcription factor containing the basic helix-loop-helix motif.

MeSH Terms
Arabidopsis/metabolism Arabidopsis Proteins Blotting, Western Cloning, Molecular Helix-Loop-Helix Motifs Phenotype Phytochrome/metabolism Phytochrome A Plant Proteins/physiology Signal Transduction/physiology
Chemicals
Arabidopsis Proteins PHYA protein, Arabidopsis Phytochrome A Plant Proteins Phytochrome
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Soh M S
Kumho Life and Environmental Science Laboratory, 1, Oryong-Dong, Puk-Gu, Kwangju 500-712, Republic of Korea. [email protected]
Kim Y M
Han S J
Song P S
References (82)
82 references, click to expand
  1. RED1 is necessary for phytochrome B-mediated red light-specific signal transduction in Arabidopsis.
    Plant Cell. 1997 May;9(5):731-43 PMID: 9165750
  2. The VLF loci, polymorphic between ecotypes Landsberg erecta and Columbia, dissect two branches of phytochrome A signal transduction that correspond to very-low-fluence and high-irradiance responses.
    Plant J. 1997 Sep;12(3):659-67 PMID: 9351250
  3. Do phytochromes interact with diverse partners?
    Trends Plant Sci. 2000 Mar;5(3):87-9 PMID: 10707071
  4. Gravity, light and plant form.
    Plant Cell Environ. 1997 Jun;20(6):796-800 PMID: 11541207
  5. Arabidopsis NPH1: a protein kinase with a putative redox-sensing domain.
    Science. 1997 Dec 19;278(5346):2120-3 PMID: 9405347
  6. The Arabidopsis HY5 gene encodes a bZIP protein that regulates stimulus-induced development of root and hypocotyl.
    Genes Dev. 1997 Nov 15;11(22):2983-95 PMID: 9367981
  7. Generation of enhancer trap lines in Arabidopsis and characterization of expression patterns in the inflorescence.
    Plant J. 1999 Mar;17(6):699-707 PMID: 10230066
  8. RAP-1 is an Arabidopsis MYC-like R protein homologue, that binds to G-box sequence motifs.
    Plant Mol Biol. 1997 May;34(1):169-74 PMID: 9177323
  9. The phytochrome apoprotein family in Arabidopsis is encoded by five genes: the sequences and expression of PHYD and PHYE.
    Plant Mol Biol. 1994 Jun;25(3):413-27 PMID: 8049367
  10. The pef mutants of Arabidopsis thaliana define lesions early in the phytochrome signaling pathway.
    Plant J. 1996 Dec;10(6):1103-10 PMID: 9011090
  11. Nuclear localization activity of phytochrome B.
    Plant J. 1996 Nov;10(5):859-68 PMID: 8953247
  12. DETECTION, ASSAY, AND PRELIMINARY PURIFICATION OF THE PIGMENT CONTROLLING PHOTORESPONSIVE DEVELOPMENT OF PLANTS.
    Proc Natl Acad Sci U S A. 1959 Dec;45(12):1703-8 PMID: 16590561
  13. Signalling in light-controlled development.
    Semin Cell Dev Biol. 1999 Apr;10(2):121-9 PMID: 10441064
  14. Nuclear targeting of the maize R protein requires two nuclear localization sequences.
    Plant Physiol. 1993 Feb;101(2):353-61 PMID: 8278504
  15. G-proteins in etiolated Avena seedlings. Possible phytochrome regulation.
    FEBS Lett. 1991 May 6;282(2):341-6 PMID: 1903719
  16. A chlorate-resistant mutant defective in the regulation of nitrate reductase gene expression in Arabidopsis defines a new HY locus.
    Plant Cell. 1997 Jan;9(1):21-35 PMID: 9014362
  17. Extragenic suppressors of the Arabidopsis det1 mutant identify elements of flowering-time and light-response regulatory pathways.
    Genetics. 1997 Apr;145(4):1125-37 PMID: 9093863
  18. Eukaryotic phytochromes: light-regulated serine/threonine protein kinases with histidine kinase ancestry.
    Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13976-81 PMID: 9811911
  19. Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family.
    Genes Dev. 1989 Nov;3(11):1745-57 PMID: 2606345
  20. Generation of expressed sequence tags of random root cDNA clones of Brassica napus by single-run partial sequencing.
    Plant Physiol. 1993 Oct;103(2):359-70 PMID: 8029332
  21. Phytochrome A null mutants of Arabidopsis display a wild-type phenotype in white light.
    Plant Cell. 1993 Jul;5(7):757-68 PMID: 8364355
  22. PKS1, a substrate phosphorylated by phytochrome that modulates light signaling in Arabidopsis.
    Science. 1999 May 28;284(5419):1539-41 PMID: 10348744
  23. fhy1 defines a branch point in phytochrome A signal transduction pathways for gene expression.
    Plant J. 1996 Dec;10(6):1155-61 PMID: 9011096
  24. Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR.
    Plant J. 1995 Sep;8(3):457-63 PMID: 7550382
  25. Cyclic GMP and calcium mediate phytochrome phototransduction.
    Cell. 1994 Apr 8;77(1):73-81 PMID: 8156599
  26. Dominant negative suppression of arabidopsis photoresponses by mutant phytochrome A sequences identifies spatially discrete regulatory domains in the photoreceptor.
    Plant Cell. 1994 Mar;6(3):449-60 PMID: 8180501
  27. Genetic and transgenic evidence that phytochromes A and B act to modulate the gravitropic orientation of Arabidopsis thaliana hypocotyls.
    Plant Physiol. 1996 Jan;110(1):211-6 PMID: 11536725
  28. The role of the COP/DET/FUS genes in light control of arabidopsis seedling development.
    Plant Physiol. 1996 Nov;112(3):871-8 PMID: 8938399
  29. Molecular interaction between COP1 and HY5 defines a regulatory switch for light control of Arabidopsis development.
    Mol Cell. 1998 Jan;1(2):213-22 PMID: 9659918
  30. Phytochrome A Mediates the Promotion of Seed Germination by Very Low Fluences of Light and Canopy Shade Light in Arabidopsis.
    Plant Physiol. 1996 Feb;110(2):439-444 PMID: 12226195
  31. Constitutive expression of the CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) gene disrupts circadian rhythms and suppresses its own expression.
    Cell. 1998 Jun 26;93(7):1207-17 PMID: 9657153
  32. Fluence and wavelength requirements for Arabidopsis CAB gene induction by different phytochromes.
    Plant Physiol. 1997 Dec;115(4):1533-40 PMID: 9414562
  33. Light quality-dependent nuclear import of the plant photoreceptors phytochrome A and B
    Plant Cell. 1999 Aug;11(8):1445-56 PMID: 10449579
  34. Attenuation of phytochrome A and B signaling pathways by the Arabidopsis circadian clock.
    Plant Cell. 1997 Oct;9(10):1727-43 PMID: 9368413
  35. Far-red light blocks greening of Arabidopsis seedlings via a phytochrome A-mediated change in plastid development.
    Plant Cell. 1996 Apr;8(4):601-15 PMID: 8624438
  36. Distinct roles for light-dependent NADPH:protochlorophyllide oxidoreductases (POR) A and B during greening in higher plants.
    Plant J. 1996 Apr;9(4):513-23 PMID: 8624514
  37. SPA1, a WD-repeat protein specific to phytochrome A signal transduction.
    Science. 1999 Apr 16;284(5413):496-9 PMID: 10205059
  38. A Myb-related transcription factor is involved in the phytochrome regulation of an Arabidopsis Lhcb gene.
    Plant Cell. 1997 Apr;9(4):491-507 PMID: 9144958
  39. Interaction between Light Quality and Light Quantity in the Photoregulation of Anthocyanin Production.
    Plant Physiol. 1990 Apr;92(4):1191-5 PMID: 16667389
  40. A simple, rapid and quantitative method for preparing Arabidopsis protein extracts for immunoblot analysis.
    Plant J. 1999 Oct;20(2):251-7 PMID: 10571885
  41. Binding of phytochrome B to its nuclear signalling partner PIF3 is reversibly induced by light.
    Nature. 1999 Aug 19;400(6746):781-4 PMID: 10466729
  42. Direct targeting of light signals to a promoter element-bound transcription factor.
    Science. 2000 May 5;288(5467):859-63 PMID: 10797009
  43. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers.
    Plant J. 1993 Aug;4(2):403-10 PMID: 8106085
  44. PIF3, a phytochrome-interacting factor necessary for normal photoinduced signal transduction, is a novel basic helix-loop-helix protein.
    Cell. 1998 Nov 25;95(5):657-67 PMID: 9845368
  45. SPA1: a new genetic locus involved in phytochrome A-specific signal transduction.
    Plant Cell. 1998 Jan;10(1):19-33 PMID: 9477570
  46. Pti4 is induced by ethylene and salicylic acid, and its product is phosphorylated by the Pto kinase.
    Plant Cell. 2000 May;12(5):771-86 PMID: 10810149
  47. Isolation and Initial Characterization of Arabidopsis Mutants That Are Deficient in Phytochrome A.
    Plant Physiol. 1993 May;102(1):269-277 PMID: 12231818
  48. Photomorphogenic development of the Arabidopsis shy2-1D mutation and its interaction with phytochromes in darkness.
    Plant J. 1998 Jul;15(1):61-8 PMID: 9744095
  49. Dynamic properties of endogenous phytochrome A in Arabidopsis seedlings.
    Plant Physiol. 1999 Oct;121(2):571-7 PMID: 10517849
  50. eid1: a new Arabidopsis mutant hypersensitive in phytochrome A-dependent high-irradiance responses.
    Plant Cell. 2000 Apr;12(4):547-58 PMID: 10760243
  51. FIN219, an auxin-regulated gene, defines a link between phytochrome A and the downstream regulator COP1 in light control of Arabidopsis development.
    Genes Dev. 2000 Aug 1;14(15):1958-70 PMID: 10921909
  52. hy8, a new class of arabidopsis long hypocotyl mutants deficient in functional phytochrome A.
    Plant Cell. 1993 Jan;5(1):39-48 PMID: 8439743
  53. Posttranslational modification of oat phytochrome A: phosphorylation of a specific serine in a multiple serine cluster.
    Biochemistry. 1997 Aug 26;36(34):10595-9 PMID: 9265641
  54. Phytochromes: photosensory perception and signal transduction.
    Science. 1995 May 5;268(5211):675-80 PMID: 7732376
  55. Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light.
    Cell. 1989 Sep 8;58(5):991-9 PMID: 2776216
  56. Targeted destabilization of HY5 during light-regulated development of Arabidopsis.
    Nature. 2000 May 25;405(6785):462-6 PMID: 10839542
  57. Phytochrome A and Phytochrome B Have Overlapping but Distinct Functions in Arabidopsis Development.
    Plant Physiol. 1994 Apr;104(4):1139-1149 PMID: 12232154
  58. ABFs, a family of ABA-responsive element binding factors.
    J Biol Chem. 2000 Jan 21;275(3):1723-30 PMID: 10636868
  59. Light: an indicator of time and place.
    Genes Dev. 2000 Feb 1;14(3):257-71 PMID: 10673498
  60. Degradation of Aux/IAA proteins is essential for normal auxin signalling.
    Plant J. 2000 Mar;21(6):553-62 PMID: 10758506
  61. Light control of plant development.
    Annu Rev Cell Dev Biol. 1997;13:203-29 PMID: 9442873
  62. Selected Components of the Shade-Avoidance Syndrome Are Displayed in a Normal Manner in Mutants of Arabidopsis thaliana and Brassica rapa Deficient in Phytochrome B.
    Plant Physiol. 1993 Aug;102(4):1179-1184 PMID: 12231894
  63. An Arabidopsis mutant hypersensitive to red and far-red light signals.
    Plant Cell. 1998 Jun;10(6):889-904 PMID: 9634578
  64. Action spectra for phytochrome A- and B-specific photoinduction of seed germination in Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):8129-33 PMID: 8755615
  65. Two dominant photomorphogenic mutations of Arabidopsis thaliana identified as suppressor mutations of hy2.
    Plant J. 1996 Apr;9(4):441-56 PMID: 8624510
  66. Eukaryotic transcription factor-DNA complexes.
    Annu Rev Biophys Biomol Struct. 1997;26:289-325 PMID: 9241421
  67. Far-red light-insensitive, phytochrome A-deficient mutants of tomato.
    Mol Gen Genet. 1995 Jan 20;246(2):133-41 PMID: 7862083
  68. Photoresponses of Light-Grown phyA Mutants of Arabidopsis (Phytochrome A Is Required for the Perception of Daylength Extensions).
    Plant Physiol. 1994 May;105(1):141-149 PMID: 12232194
  69. Cryptochromes: blue light receptors for plants and animals.
    Science. 1999 Apr 30;284(5415):760-5 PMID: 10221900
  70. PAT1, a new member of the GRAS family, is involved in phytochrome A signal transduction.
    Genes Dev. 2000 May 15;14(10):1269-78 PMID: 10817761
  71. Missense mutations define a restricted segment in the C-terminal domain of phytochrome A critical to its regulatory activity.
    Plant Cell. 1995 Sep;7(9):1433-43 PMID: 8589627
  72. Genetic identification of FIN2, a far red light-specific signaling component of Arabidopsis thaliana.
    Plant J. 1998 Nov;16(4):411-9 PMID: 9881161
  73. Phytochrome a overexpression inhibits hypocotyl elongation in transgenic Arabidopsis.
    Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10806-10 PMID: 11607244
  74. Carboxy-terminal deletion analysis of oat phytochrome A reveals the presence of separate domains required for structure and biological activity.
    Plant Cell. 1993 May;5(5):565-75 PMID: 8518556
  75. Functional analysis of yeast-derived phytochrome A and B phycocyanobilin adducts.
    Plant J. 1996 Oct;10(4):625-36 PMID: 8893541
  76. Light-dependent translocation of a phytochrome B-GFP fusion protein to the nucleus in transgenic Arabidopsis.
    J Cell Biol. 1999 May 3;145(3):437-45 PMID: 10225946
  77. The FAR1 locus encodes a novel nuclear protein specific to phytochrome A signaling.
    Genes Dev. 1999 Aug 1;13(15):2017-27 PMID: 10444599
  78. Identification by large-scale screening of phytochrome-regulated genes in etiolated seedlings of Arabidopsis using a fluorescent differential display technique.
    Plant Physiol. 2000 Jan;122(1):15-24 PMID: 10631245
  79. The light-induced reduction of the gravitropic growth-orientation of seedlings of Arabidopsis thaliana (L.) Heynh. is a photomorphogenic response mediated synergistically by the far-red-absorbing forms of phytochromes A and B.
    Planta. 1996;199(4):511-4 PMID: 8818290
  80. Both phyA and phyB mediate light-imposed repression of PHYA gene expression in Arabidopsis.
    Plant Physiol. 1999 Dec;121(4):1207-16 PMID: 10594107
  81. Phytochrome signalling is mediated through nucleoside diphosphate kinase 2.
    Nature. 1999 Oct 7;401(6753):610-3 PMID: 10524631
  82. Calcium/calmodulin-dependent and -independent phytochrome signal transduction pathways.
    Cell. 1993 Jun 4;73(5):937-52 PMID: 8388782
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.12.11.2061
Email
[email protected]
Published
2000-11-00
Pages
2061-74
Language
English
Region
England
NLM ID
9208688
PMCID
PMC150158
Subset
IM
Grants
NIGMS NIH HHS · GM36956 · United States
External Links
PubMed source DOI: 10.1105/tpc.12.11.2061 PMC Full Text View journal details
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