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PMID: 11139489 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two related proteins, Edc1p and Edc2p, stimulate mRNA decapping in Saccharomyces cerevisiae.

Genetics ·Vol. 157 ·No. 1 ·2001-01-00 ·Pages 27-37

Dunckley T, Tucker M, Parker R

Abstract

The major mRNA decay pathway in Saccharomyces cerevisiae occurs through deadenylation, decapping, and 5' to 3' degradation of the mRNA. Decapping is a critical control point in this decay pathway. Two proteins, Dcp1p and Dcp2p, are required for mRNA decapping in vivo and for the production of active decapping enzyme. To understand the relationship between Dcp1p and Dcp2p, a combination of both genetic and biochemical approaches were used. First, we demonstrated that when Dcp1p is biochemically separated from Dcp2p, Dcp1p was active for decapping. This observation confirmed that Dcp1p is the decapping enzyme and indicated that Dcp2p functions to allow the production of active Dcp1p. We also identified two related proteins that stimulate decapping, Edc1p and Edc2p (Enhancer of mRNA DeCapping). Overexpression of the EDC1 and EDC2 genes suppressed conditional alleles of dcp1 and dcp2, respectively. Moreover, when mRNA decapping was compromised, deletion of the EDC1 and/or EDC2 genes caused significant mRNA decay defects. The Edc1p also co-immunoprecipitated with Dcp1p and Dcp2p. These results indicated that Edc1p and Edc2p interact with the decapping proteins and function to enhance the decapping rate.

MeSH Terms
Alleles Amino Acid Sequence Fungal Proteins/genetics,metabolism Gene Deletion Genes, Fungal Molecular Sequence Data RNA Caps/metabolism RNA, Fungal/metabolism RNA, Messenger/metabolism RNA-Binding Proteins Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid
Chemicals
Edc1 protein, S cerevisiae Edc2 protein, S cerevisiae Fungal Proteins RNA Caps RNA, Fungal RNA, Messenger RNA-Binding Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dunckley T
Department of Molecular and Cellular Biology and Howard Hughes Medical Institute, University of Arizona, Tucson, Arizona 85721, USA.
Tucker M
Parker R
References (33)
33 references, click to expand
  1. Deadenylation of the unstable mRNA encoded by the yeast MFA2 gene leads to decapping followed by 5'-->3' digestion of the transcript.
    Genes Dev. 1994 Apr 1;8(7):855-66 PMID: 7926773
  2. Premature translational termination triggers mRNA decapping.
    Nature. 1994 Aug 18;370(6490):578-81 PMID: 8052314
  3. Identification and characterization of a human cDNA homologous to yeast SKI2.
    Genomics. 1995 Feb 10;25(3):660-6 PMID: 7759100
  4. Human helicase gene SKI2W in the HLA class III region exhibits striking structural similarities to the yeast antiviral gene SKI2 and to the human gene KIAA0052: emergence of a new gene family.
    Nucleic Acids Res. 1995 Jun 25;23(12):2120-6 PMID: 7610041
  5. mRNA stability in mammalian cells.
    Microbiol Rev. 1995 Sep;59(3):423-50 PMID: 7565413
  6. An essential component of the decapping enzyme required for normal rates of mRNA turnover.
    Nature. 1996 Aug 15;382(6592):642-6 PMID: 8757137
  7. Interrelationships of the pathways of mRNA decay and translation in eukaryotic cells.
    Annu Rev Biochem. 1996;65:693-739 PMID: 8811193
  8. The MutT proteins or "Nudix" hydrolases, a family of versatile, widely distributed, "housecleaning" enzymes.
    J Biol Chem. 1996 Oct 11;271(41):25059-62 PMID: 8810257
  9. A Sm-like protein complex that participates in mRNA degradation.
    EMBO J. 2000 Apr 3;19(7):1661-71 PMID: 10747033
  10. Yeast Sm-like proteins function in mRNA decapping and decay.
    Nature. 2000 Mar 30;404(6777):515-8 PMID: 10761922
  11. A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing.
    EMBO J. 2000 Apr 17;19(8):1873-86 PMID: 10775271
  12. Function of the ski4p (Csl4p) and Ski7p proteins in 3'-to-5' degradation of mRNA.
    Mol Cell Biol. 2000 Nov;20(21):8230-43 PMID: 11027292
  13. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  14. A quantitative analysis of the effects of 5' junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing.
    Cell. 1985 Dec;43(2 Pt 1):423-30 PMID: 3935321
  15. The rate-limiting step in yeast PGK1 mRNA degradation is an endonucleolytic cleavage in the 3'-terminal part of the coding region.
    Mol Cell Biol. 1992 Jul;12(7):2986-96 PMID: 1320194
  16. Human beta-globin mRNAs that harbor a nonsense codon are degraded in murine erythroid tissues to intermediates lacking regions of exon I or exons I and II that have a cap-like structure at the 5' termini.
    EMBO J. 1992 Sep;11(9):3271-8 PMID: 1324170
  17. Yeast cells lacking 5'-->3' exoribonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5' cap structure.
    Mol Cell Biol. 1993 Aug;13(8):4826-35 PMID: 8336719
  18. A turnover pathway for both stable and unstable mRNAs in yeast: evidence for a requirement for deadenylation.
    Genes Dev. 1993 Aug;7(8):1632-43 PMID: 8393418
  19. A small segment of the MAT alpha 1 transcript promotes mRNA decay in Saccharomyces cerevisiae: a stimulatory role for rare codons.
    Mol Cell Biol. 1993 Sep;13(9):5141-8 PMID: 8355674
  20. A highly conserved sequence motif defining the family of MutT-related proteins from eubacteria, eukaryotes and viruses.
    Nucleic Acids Res. 1993 Oct 11;21(20):4847 PMID: 8233837
  21. Mutations in trans-acting factors affecting mRNA decapping in Saccharomyces cerevisiae.
    Mol Cell Biol. 1996 Oct;16(10):5830-8 PMID: 8816497
  22. Mechanisms and control of mRNA turnover in Saccharomyces cerevisiae.
    Microbiol Rev. 1996 Mar;60(1):233-49 PMID: 8852902
  23. A mouse cytoplasmic exoribonuclease (mXRN1p) with preference for G4 tetraplex substrates.
    J Cell Biol. 1997 Feb 24;136(4):761-73 PMID: 9049243
  24. Messenger RNA deadenylylation precedes decapping in mammalian cells.
    Proc Natl Acad Sci U S A. 1997 May 27;94(11):5628-33 PMID: 9159123
  25. The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases.
    Cell. 1997 Nov 14;91(4):457-66 PMID: 9390555
  26. Isolation and characterization of Dcp1p, the yeast mRNA decapping enzyme.
    EMBO J. 1998 Mar 2;17(5):1487-96 PMID: 9482745
  27. Capped mRNA degradation intermediates accumulate in the yeast spb8-2 mutant.
    Mol Cell Biol. 1998 Sep;18(9):5062-72 PMID: 9710590
  28. Analysis of mRNA decay pathways in Saccharomyces cerevisiae.
    Methods. 1999 Jan;17(1):3-10 PMID: 10075877
  29. Analysis of mutations in the yeast mRNA decapping enzyme.
    Genetics. 1999 Apr;151(4):1273-85 PMID: 10101156
  30. Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin.
    EMBO J. 1999 Jun 15;18(12):3451-62 PMID: 10369684
  31. Mutations in translation initiation factors lead to increased rates of deadenylation and decapping of mRNAs in Saccharomyces cerevisiae.
    Mol Cell Biol. 1999 Aug;19(8):5247-56 PMID: 10409716
  32. The DCP2 protein is required for mRNA decapping in Saccharomyces cerevisiae and contains a functional MutT motif.
    EMBO J. 1999 Oct 1;18(19):5411-22 PMID: 10508173
  33. Characterization of cis-acting sequences and decay intermediates involved in nonsense-mediated mRNA turnover.
    Mol Cell Biol. 1995 Feb;15(2):809-23 PMID: 7823948
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/157.1.27
Published
2001-01-00
Pages
27-37
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461477
Subset
IM
Grants
NIGMS NIH HHS · GM45443 · United States
External Links
PubMed source DOI: 10.1093/genetics/157.1.27 PMC Full Text View journal details
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