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PMID: 11149932 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Sar1 GTPase coordinates biosynthetic cargo selection with endoplasmic reticulum export site assembly.

The Journal of cell biology ·Vol. 152 ·No. 1 ·2001-01-08 ·Pages 213-29

Aridor M, Fish KN, Bannykh S, Weissman J, Roberts TH, Lippincott-Schwartz J, Balch WE

Abstract

Cargo selection and export from the endoplasmic reticulum is mediated by the COPII coat machinery that includes the small GTPase Sar1 and the Sec23/24 and Sec13/31 complexes. We have analyzed the sequential events regulated by purified Sar1 and COPII coat complexes during synchronized export of cargo from the ER in vitro. We find that activation of Sar1 alone, in the absence of other cytosolic components, leads to the formation of ER-derived tubular domains that resemble ER transitional elements that initiate cargo selection. These Sar1-generated tubular domains were shown to be transient, functional intermediates in ER to Golgi transport in vitro. By following cargo export in live cells, we show that ER export in vivo is also characterized by the formation of dynamic tubular structures. Our results demonstrate an unanticipated and novel role for Sar1 in linking cargo selection with ER morphogenesis through the generation of transitional tubular ER export sites.

MeSH Terms
Animals Biological Transport COP-Coated Vesicles/metabolism Cytoplasm/metabolism Endoplasmic Reticulum/metabolism Enzyme Activation Fluorescence Golgi Apparatus/metabolism Intracellular Membranes/metabolism Membrane Glycoproteins Microscopy, Video Monomeric GTP-Binding Proteins/metabolism Saccharomyces cerevisiae Proteins Time Factors Vesicular Transport Proteins Viral Envelope Proteins/metabolism
Chemicals
G protein, vesicular stomatitis virus Membrane Glycoproteins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins Viral Envelope Proteins Monomeric GTP-Binding Proteins SAR1 protein, S cerevisiae
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Aridor M
Department of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Fish K N
Bannykh S
Weissman J
Roberts T H
Lippincott-Schwartz J
Balch W E
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.152.1.213
Published
2001-01-08
Pages
213-29
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2193666
Subset
IM
Grants
NIGMS NIH HHS · GM42336 · United States
NCI NIH HHS · CA58689 · United States
NIGMS NIH HHS · F32 GM019689-01A1 · United States
NIGMS NIH HHS · F32 GM019689 · United States
NIDDK NIH HHS · R01 DK062318 · United States
NIGMS NIH HHS · F32 GM019689-03 · United States
NIGMS NIH HHS · F32 GM019689-02 · United States
External Links
PubMed source DOI: 10.1083/jcb.152.1.213 PMC Full Text View journal details
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