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PMID: 11159908 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

E2Fs regulate the expression of genes involved in differentiation, development, proliferation, and apoptosis.

Genes & development ·Vol. 15 ·No. 3 ·2001-02-01 ·Pages 267-85

Müller H, Bracken AP, Vernell R, Moroni MC, Christians F, Grassilli E, Prosperini E, Vigo E, Oliner JD, Helin K

Abstract

The retinoblastoma protein (pRB) and its two relatives, p107 and p130, regulate development and cell proliferation in part by inhibiting the activity of E2F-regulated promoters. We have used high-density oligonucleotide arrays to identify genes in which expression changed in response to activation of E2F1, E2F2, and E2F3. We show that the E2Fs control the expression of several genes that are involved in cell proliferation. We also show that the E2Fs regulate a number of genes involved in apoptosis, differentiation, and development. These results provide possible genetic explanations to the variety of phenotypes observed as a consequence of a deregulated pRB/E2F pathway.

MeSH Terms
Apoptosis/genetics Blotting, Northern Carrier Proteins Cell Cycle/genetics Cell Cycle Proteins Cell Differentiation/genetics Cell Division/genetics DNA Replication DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor E2F2 Transcription Factor E2F3 Transcription Factor Gene Expression Profiling Gene Expression Regulation Gene Targeting Humans Oligonucleotide Array Sequence Analysis Protein Isoforms/physiology Retinoblastoma Protein/physiology Retinoblastoma-Binding Protein 1 Transcription Factor DP1 Transcription Factors/physiology Tumor Cells, Cultured
Chemicals
Carrier Proteins Cell Cycle Proteins DNA-Binding Proteins E2F Transcription Factors E2F1 Transcription Factor E2F1 protein, human E2F2 Transcription Factor E2F2 protein, human E2F3 Transcription Factor Protein Isoforms Retinoblastoma Protein Retinoblastoma-Binding Protein 1 Transcription Factor DP1 Transcription Factors
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Müller H
Department of Experimental Oncology, European Institute of Oncology, 20141 Milan, Italy.
Bracken A P
Vernell R
Moroni M C
Christians F
Grassilli E
Prosperini E
Vigo E
Oliner J D
Helin K
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.864201
Published
2001-02-01
Pages
267-85
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312619
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.864201 PMC Full Text View journal details
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