Home LiteratureArticle Details
PMID: 11160122 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

VibD and VibH are required for late steps in vibriobactin biosynthesis in Vibrio cholerae.

Journal of bacteriology ·Vol. 183 ·No. 5 ·2001-03-00 ·Pages 1830-4

Wyckoff EE, Smith SL, Payne SM

Abstract

Vibrio cholerae synthesizes the catechol siderophore vibriobactin. In this report, we present the complete map of a vibriobactin gene region containing two previously unreported vibriobactin biosynthetic genes. vibD encodes a phosphopantetheinyl transferase, and vibH encodes a novel nonribosomal peptide synthase. Both VibD and VibH are required for vibriobactin biosynthesis.

MeSH Terms
Bacterial Proteins/genetics,metabolism Catechols/chemistry,metabolism Enterobactin/metabolism Genes, Bacterial Molecular Sequence Data Multigene Family Oxazoles Peptide Synthases/genetics,metabolism Plasmids Transferases (Other Substituted Phosphate Groups)/genetics,metabolism Vibrio cholerae/enzymology,genetics
Chemicals
Bacterial Proteins Catechols Oxazoles Enterobactin vibriobactin Transferases (Other Substituted Phosphate Groups) holo-(acyl-carrier-protein) synthase Peptide Synthases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wyckoff E E
Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712-1095.
Smith S L
Payne S M
References (29)
29 references, click to expand
  1. A multifunctional ATP-binding cassette transporter system from Vibrio cholerae transports vibriobactin and enterobactin.
    J Bacteriol. 1999 Dec;181(24):7588-96 PMID: 10601218
  2. N-Acyl-L-homoserine lactone autoinducers control production of an extracellular lipopeptide biosurfactant required for swarming motility of Serratia liquefaciens MG1.
    J Bacteriol. 1998 Dec;180(23):6384-8 PMID: 9829950
  3. Identification of an operon required for ferrichrome iron utilization in Vibrio cholerae.
    J Bacteriol. 2000 Apr;182(8):2350-3 PMID: 10735886
  4. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae.
    Nature. 2000 Aug 3;406(6795):477-83 PMID: 10952301
  5. Effect of iron limitation on growth, siderophore production, and expression of outer membrane proteins of Vibrio cholerae.
    J Bacteriol. 1982 Apr;150(1):148-55 PMID: 6460753
  6. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  7. Vibriobactin, a siderophore from Vibrio cholerae.
    J Biol Chem. 1984 Jan 10;259(1):383-5 PMID: 6706943
  8. The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide sequence and membrane localization of its protein product.
    Mol Microbiol. 1989 Jun;3(6):757-66 PMID: 2526281
  9. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  10. Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine.
    Biochemistry. 1991 Mar 19;30(11):2916-27 PMID: 1826089
  11. Further study on polyamine compositions in Vibrionaceae.
    Can J Microbiol. 1991 Feb;37(2):148-53 PMID: 2059921
  12. Identification of the vibriobactin receptor of Vibrio cholerae.
    J Bacteriol. 1992 May;174(10):3270-4 PMID: 1315733
  13. Cloning, sequencing, and transcriptional regulation of viuA, the gene encoding the ferric vibriobactin receptor of Vibrio cholerae.
    J Bacteriol. 1992 Jun;174(11):3729-38 PMID: 1317381
  14. Cloning and characterization of the Vibrio cholerae genes encoding the utilization of iron from haemin and haemoglobin.
    Mol Microbiol. 1993 Feb;7(3):461-9 PMID: 8384684
  15. MacVector: an integrated sequence analysis program for the Macintosh.
    Methods Mol Biol. 1994;25:195-201 PMID: 8004165
  16. Identification, cloning, and sequencing of a gene required for ferric vibriobactin utilization by Vibrio cholerae.
    J Bacteriol. 1994 Sep;176(18):5631-8 PMID: 8083157
  17. Modular structure of genes encoding multifunctional peptide synthetases required for non-ribosomal peptide synthesis.
    FEMS Microbiol Lett. 1995 Jan 1;125(1):3-14 PMID: 7867917
  18. Multienzymatic non ribosomal peptide biosynthesis: identification of the functional domains catalysing peptide elongation and epimerisation.
    C R Acad Sci III. 1995 Sep;318(9):927-36 PMID: 8521076
  19. A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome.
    Mol Microbiol. 1996 Jul;21(1):77-96 PMID: 8843436
  20. Sequence and genetic organization of a Bacillus subtilis operon encoding 2,3-dihydroxybenzoate biosynthetic enzymes.
    Gene. 1996 Oct 31;178(1-2):119-23 PMID: 8921902
  21. A new enzyme superfamily - the phosphopantetheinyl transferases.
    Chem Biol. 1996 Nov;3(11):923-36 PMID: 8939709
  22. Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate.
    Biochemistry. 1997 Jul 15;36(28):8495-503 PMID: 9214294
  23. Cloning of a Vibrio cholerae vibriobactin gene cluster: identification of genes required for early steps in siderophore biosynthesis.
    J Bacteriol. 1997 Nov;179(22):7055-62 PMID: 9371453
  24. Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntF.
    Biochemistry. 1998 Feb 24;37(8):2648-59 PMID: 9485415
  25. Molecular cloning of the actinomycin synthetase gene cluster from Streptomyces chrysomallus and functional heterologous expression of the gene encoding actinomycin synthetase II.
    J Bacteriol. 1998 May;180(9):2468-74 PMID: 9573200
  26. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic role of the condensation domain.
    J Biol Chem. 1998 Aug 28;273(35):22773-81 PMID: 9712910
  27. Vibrio cholerae iron transport: haem transport genes are linked to one of two sets of tonB, exbB, exbD genes.
    Mol Microbiol. 1998 Sep;29(6):1493-507 PMID: 9781885
  28. The Vibrio cholerae genome contains two unique circular chromosomes.
    Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14464-9 PMID: 9826723
  29. Vibrio cholerae VibF is required for vibriobactin synthesis and is a member of the family of nonribosomal peptide synthetases.
    J Bacteriol. 2000 Mar;182(6):1731-8 PMID: 10692380
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.183.5.1830-1834.2001
Published
2001-03-00
Pages
1830-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC95076
Subset
IM
Grants
NIAID NIH HHS · R01 AI016935 · United States
NIAID NIH HHS · R37 AI016935 · United States
NIAID NIH HHS · AI16935 · United States
Databases
GENBANK
U52150
External Links
PubMed source DOI: 10.1128/JB.183.5.1830-1834.2001 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2