Abstract
To redirect the tropism of the vaccine strain of measles virus (MV), Edmonston B, to a targeted cell population, we displayed on the viral hemagglutinin (H) a single-chain antibody (scAb) specific for the tumor-associated carcinoembryonic antigen (CEA). We generated H fusion proteins with three forms of the scAb appended, differing in the lengths of the linkers separating the VH and VL domains and thus in the oligomerization states of the scAbs. All proteins were stable, appeared properly folded, and were transported to the cell surface, but only H displaying the long-linker form of scAb was functional in supporting cell-cell fusion. This protein induced extensive syncytia in cells expressing the normal virus receptor CD46 and also in CD46-negative cells expressing the targeted receptor, human CEA. Replication-competent MV with H replaced by H displaying the long-linker form of scAb was recovered and replicated efficiently in both CD46-positive and CD46-negative, CEA-positive cells. Thus, MV not only tolerates the addition of a scAb on its H protein but also infects cells via a novel interaction between the scAb and its targeted receptor.
MeSH Terms
Animals
Antibodies/genetics,immunology,metabolism
Antigens, CD/analysis
Carcinoembryonic Antigen/immunology,metabolism
Cell Line
Flow Cytometry
Giant Cells/physiology
Hemagglutinins, Viral/genetics,immunology,metabolism
Humans
Measles virus/genetics,immunology,physiology
Membrane Cofactor Protein
Membrane Glycoproteins/analysis
Recombinant Fusion Proteins/metabolism
Recombination, Genetic
Transfection
Chemicals
Antibodies
Antigens, CD
CD46 protein, human
Carcinoembryonic Antigen
Hemagglutinins, Viral
Membrane Cofactor Protein
Membrane Glycoproteins
Recombinant Fusion Proteins
hemagglutinin protein G, measles virus
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hammond A L
Molecular Medicine Program, Mayo Foundation, Rochester, Minnesota 55905, USA.
Plemper R K
Zhang J
Schneider U
Russell S J
Cattaneo R
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