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PMID: 11179307 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

cDNA array analysis of altered gene expression in human endothelial cells in response to Chlamydia pneumoniae infection.

Infection and immunity ·Vol. 69 ·No. 3 ·2001-03-00 ·Pages 1420-7

Coombes BK, Mahony JB

Abstract

Strong epidemiological and pathological evidence supports a role for Chlamydia pneumoniae infection in atherosclerosis and human coronary heart disease. Animal models have shown that C. pneumoniae disseminates hematogenously in infected monocytes and macrophages, while in vitro data suggest that infected macrophages can transmit C. pneumoniae infection directly to endothelial cells. Endothelial cells may be key in vivo targets for C. pneumoniae infection; given that these cells are important in regulating the dynamics of the vessel wall, we used cDNA microarrays to study the transcriptional response of endothelial cells to infection with C. pneumoniae. cDNA arrays were used to characterize the mRNA expression profiles for 268 human genes following infection with C. pneumoniae, which were compared to mRNA profiles of uninfected cells. Selected genes of interest were further investigated by reverse transcription-PCR throughout a 24-h period of infection. C. pneumoniae infection upregulated mRNA expression for approximately 20 (8%) of the genes studied. Genes coding for cytokines (interleukin-1), chemokines (monocyte chemotactic protein 1 and interleukin-8), and cellular growth factors (heparin-binding epidermal-like growth factor, basic fibroblast growth factor, and platelet-derived growth factor B chain) were the most prominently upregulated. In addition to these families of genes, increases in mRNA levels for intracellular kinases and cell surface receptors with signal transduction activities were observed. Time course experiments showed that mRNA levels were upregulated within 2 h following infection. These results expand our knowledge of the response of endothelial cells to C. pneumoniae by further defining the repertoire of C. pneumoniae-inducible genes and provide new insight into potential mechanisms of atherogenesis. In addition, the use of cDNA microarrays may prove useful for the study of host cell responses to C. pneumoniae infection during latent and replicative stages of infection and related pathology.

MeSH Terms
Arteriosclerosis/etiology Chlamydophila Infections/genetics Chlamydophila pneumoniae Coronary Disease/etiology DNA, Complementary/isolation & purification Endothelium, Vascular/microbiology Gene Expression Profiling Humans Oligonucleotide Array Sequence Analysis/methods RNA, Messenger/analysis Reverse Transcriptase Polymerase Chain Reaction
Chemicals
DNA, Complementary RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coombes B K
Department of Medical Sciences, McMaster University, and Father Sean O'Sullivan Research Centre, St. Joseph's Hospital, Hamilton, Ontario, Canada L8N [email protected]
Mahony J B
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.69.3.1420-1427.2001
Email
[email protected]
Published
2001-03-00
Pages
1420-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC98036
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.69.3.1420-1427.2001 PMC Full Text View journal details
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