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PMID: 11179350 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and characterization of a second extracellular collagen-like protein made by group A Streptococcus: control of production at the level of translation.

Infection and immunity ·Vol. 69 ·No. 3 ·2001-03-00 ·Pages 1729-38

Lukomski S, Nakashima K, Abdi I, Cipriano VJ, Shelvin BJ, Graviss EA, Musser JM

Abstract

A recent study found that group A Streptococcus (GAS) expresses a cell surface protein with similarity to human collagen (S. Lukomski, K. Nakashima, I. Abdi, V. J. Cipriano, R. M. Ireland, S. R. Reid, G. G. Adams, and J. M. Musser, Infect. Immun. 68:6542-6553, 2000). This streptococcal collagen-like protein (Scl) contains a long region of Gly-X-X motifs and was produced by serotype M1 GAS strains. In the present study, a second member of the scl gene family was identified and designated scl2. The Scl2 protein also has a collagen-like region, which in M1 strains is composed of 38 contiguous Gly-X-X triplet motifs. The scl2 gene was present in all 50 genetically diverse GAS strains studied. The Scl2 protein is highly polymorphic, and the number of Gly-X-X motifs in the 50 strains studied ranged from 31 in one serotype M1 strain to 79 in serotype M28 and M77 isolates. The scl1 and scl2 genes were simultaneously transcribed in the exponential phase, and the Scl proteins were also produced. Scl1 and Scl2 were identified in a cell-associated form and free in culture supernatants. Production of Scl1 is regulated by Mga, a positive transcriptional regulator that controls expression of several GAS virulence factors. In contrast, production of Scl2 is controlled at the level of translation by variation in the number of short-sequence pentanucleotide repeats (CAAAA) located immediately downstream of the GTG (Val) start codon. Control of protein production by this molecular mechanism has not been identified previously in GAS. Together, the data indicate that GAS simultaneously produces two extracellular human collagen-like proteins in a regulated fashion.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/metabolism Base Sequence Collagen/genetics,metabolism Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Protein Biosynthesis Sequence Homology, Amino Acid Streptococcus pyogenes/genetics Transcription Factors/metabolism

Chemicals

Bacterial Proteins Scl1 protein, Streptococcus Scl2 protein, Streptococcus Transcription Factors mry protein, Streptococcus pyogenes Collagen

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Lukomski S

Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA.

Nakashima K

Abdi I

Cipriano V J

Shelvin B J

Graviss E A

Musser J M

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/IAI.69.3.1729-1738.2001

Published

2001-03-00

Pages

1729-38

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC98079

Subset

Grants

NIAID NIH HHS · AI-33119 · United States

NIAID NIH HHS · AI38406 · United States

Databases

GENBANK

AF317835

External Links

PubMed source DOI: 10.1128/IAI.69.3.1729-1738.2001 PMC Full Text View journal details

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Identification and characterization of a second extracellular collagen-like protein made by group A Streptococcus: control of production at the level of translation.

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