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PMID: 11198425 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Circadian and senescence-enhanced expression of a tobacco cysteine protease gene.

Plant molecular biology ·Vol. 44 ·No. 5 ·2000-11-00 ·Pages 649-57

Ueda T, Seo S, Ohashi Y, Hashimoto J

Abstract

A cDNA clone encoding a cysteine protease was isolated from a tobacco cDNA library, utilizing as a probe a PCR fragment obtained from degenerated primers based on the conserved sequences of plant cysteine protease genes. A putative protein encoded by the clone NTCP-23 had an amino acid sequence with significant similarities to those of plant senescence-associated cysteine proteases and mammalian cathepsin H. Northern blot analysis showed that NTCP-23 mRNA is expressed in all organs and the mRNA and protein expression is enhanced during natural senescence. We propose that NTCP-23 is responsible for amino acid remobilization especially in senescencing leaves. Furthermore, it was found that the mRNA expression follows a circadian rhythm and is reduced by continuous darkness, wounding and hypersensitive reaction (HR). NTCP-23 is the first cysteine protease whose mRNA expression has been shown to be temporarily reduced by wounding.

MeSH Terms
Amino Acid Sequence Blotting, Northern Circadian Rhythm/physiology Cloning, Molecular Cysteine Endopeptidases/genetics,metabolism DNA, Complementary/chemistry,genetics Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Phylogeny Plant Diseases/genetics,virology Plant Leaves/enzymology,genetics Plants, Toxic RNA, Messenger/genetics,metabolism RNA, Plant/genetics,metabolism Sequence Analysis, DNA Sequence Homology, Amino Acid Stress, Mechanical Tissue Distribution Tobacco/enzymology,genetics,growth & development Tobacco Mosaic Virus/growth & development
Chemicals
DNA, Complementary RNA, Messenger RNA, Plant Cysteine Endopeptidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ueda T
National Institute of Agrobiological Resources, Tsukuba, Ibaraki, Japan. [email protected]
Seo S
Ohashi Y
Hashimoto J
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1026546004942
Email
[email protected]
Published
2000-11-00
Pages
649-57
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
AB032168
External Links
PubMed source DOI: 10.1023/a:1026546004942 View journal details
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