Lymphocyte differentiation and antibody synthesis in the secondary immune response of peroxidase stimulated lymph nodes of rat.

Cell and tissue research ·Vol. 156 ·No. 3 ·1975-00-00 ·Pages 391-402

Kuhlmann WD, Avrameas S

Abstract

Lymphocyte differentiation and specific antibody synthesis were studied in the course of the secondary immune response of horseradish-peroxidase immunized rat popliteal lymph nodes by electron microscopic immunocytochemistry. From primary immunization, mature plasma cells containing specific antibody remained as long as 10 months. Antihorseradish peroxidase antibodies were localized in lymphocytic blast cells on Day 3 after restimulation with antigen. In the subsequent days blast cells were still present, and shifting to mature stages occured. During immunocyte differentiation and maturation vesicles and tubules of the Golgi complex became filled with specific antibody suggesting occasional secretion of antibody via the Golgi apparatus. At very mature cell stages, antibody was accumulated in distended cisternae.

MeSH Terms

Animals Antibody Formation Cell Differentiation Endoplasmic Reticulum/immunology Golgi Apparatus/immunology Immunity Immunization Schedule Immunization, Secondary Lymph Nodes/drug effects,immunology Lymphocytes/immunology Microscopy, Electron Peroxidases/administration & dosage,immunology Plasma Cells/immunology Rats

Chemicals

Peroxidases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kuhlmann W D

Avrameas S

References (13)

13 references, click to expand

Improvements in epoxy resin embedding methods.
J Biophys Biochem Cytol. 1961 Feb;9:409-14 PMID: 13764136
LOCALIZATION OF ANTIBODIES BY ELECTRON MICROSCOPY IN DEVELOPING ANTIBODY-PRODUCING CELLS.
J Cell Biol. 1965 Sep 1;26(3):759-78 PMID: 19866679
A comparative study of the techniques for ultrastructural localization of antienzyme antibodies.
J Ultrastruct Res. 1971 May;35(3):370-85 PMID: 4104249
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
J Histochem Cytochem. 1966 Apr;14(4):291-302 PMID: 5962951
The proportion and structure of cells forming antibody, gamma G and gamma M immunoglobulins, and gamma G and gamma M antibodies.
Cell Immunol. 1970 May;1(1):31-50 PMID: 5002228
Localization of antibodies in plasma cells by electron microscopy.
J Exp Med. 1963 May 1;117:849-62 PMID: 14026001
Location of antibody to horseradish peroxidase in popliteal lymph nodes of rabbits during the primary and early secondary response.
J Histochem Cytochem. 1970 Feb;18(2):120-30 PMID: 4190152
Detection of simultaneous antibody synthesis in plasma cells and specialized lymphocytes in rabbit lymph nodes.
J Exp Med. 1970 Jun 1;131(6):1137-68 PMID: 5419269
The carriage of immunological memory by small lymphocytes in the rat.
J Exp Med. 1966 Nov 1;124(5):1017-30 PMID: 5926297
Cellular differentiation and antibody localization during the primary immune response in peroxidase stimulated lymph nodes of rat.
Cell Immunol. 1972 Aug;4(4):425-41 PMID: 5065737
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
J Cell Biol. 1963 Apr;17:208-12 PMID: 13986422
Ultrastructural localization of antibody in differentiating plasma cells.
J Exp Med. 1968 Jan 1;127(1):109-18 PMID: 5635036
Specific antibody within lymphoid germinal center cells of mice after primary immunization with horseradish peroxidase: a light and electron microscopic study.
J Exp Med. 1970 Jan 1;131(1):77-91 PMID: 5409949

Article Info

Journal

Cell and tissue research

Abbr.

Cell Tissue Res

ISSN

0302-766X

DOI

10.1007/BF00225367

Published

1975-00-00

Pages

391-402

Language

English

Region

Germany

NLM ID

0417625

Subset

External Links

PubMed source DOI: 10.1007/BF00225367 View journal details

Analysis Services

Lymphocyte differentiation and antibody synthesis in the secondary immune response of peroxidase stimulated lymph nodes of rat.

Contact

WeChat Official Account