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PMID: 11230148 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Putative telomere-independent mechanisms of replicative aging reflect inadequate growth conditions.

Genes & development ·Vol. 15 ·No. 4 ·2001-02-15 ·Pages 398-403

Ramirez RD, Morales CP, Herbert BS, Rohde JM, Passons C, Shay JW, Wright WE

Abstract

Telomere shortening is the mechanism underlying replicative aging in fibroblasts. A variety of reports now claim that inactivation of the p16(INK4a)/pRB pathway is required in addition to telomere maintenance for the immortalization of cells such as skin keratinocytes and breast epithelial cells. We here show that the premature growth arrest of these cell types can be explained by an inadequate culture environment. Providing mesenchymal/epithelial interactions by cultivating the telomerase-expressing cells on feeder layers avoids the growth arrest associated with increased p16(INK4a). These results do not support a telomere-independent mechanism of replicative aging.

MeSH Terms
3T3 Cells Animals Cell Division Cell Line, Transformed Cellular Senescence/genetics Culture Media Cyclin-Dependent Kinase Inhibitor p16/biosynthesis Epithelial Cells/cytology Female Fibroblasts/cytology Humans Keratinocytes/cytology,enzymology Mammary Glands, Animal/cytology Mice Skin/cytology Telomerase/metabolism Telomere
Chemicals
Culture Media Cyclin-Dependent Kinase Inhibitor p16 Telomerase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ramirez R D
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9039, USA.
Morales C P
Herbert B S
Rohde J M
Passons C
Shay J W
Wright W E
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.859201
Published
2001-02-15
Pages
398-403
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC312628
Subset
IM
Grants
NIA NIH HHS · R01 AG001228 · United States
NIA NIH HHS · AG01228 · United States
External Links
PubMed source DOI: 10.1101/gad.859201 PMC Full Text View journal details
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