Home LiteratureArticle Details

PMID: 11237468 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of dharma/bozozok by the Wnt pathway.

Developmental biology ·Vol. 231 ·No. 2 ·2001-03-15 ·Pages 397-409

Ryu SL, Fujii R, Yamanaka Y, Shimizu T, Yabe T, Hirata T, Hibi M, Hirano T

Abstract

The zebrafish homeobox gene dharma/bozozok (boz) is required for the formation and/or function of the Nieuwkoop center and the subsequent induction of the Spemann organizer. dharma is expressed soon after the midblastula transition in the dorsal blastomeres and the dorsal yolk syncytial layer (YSL). We found that the expression of dharma was upregulated or ectopically induced by misexpression of a Wnt protein and cytoplasmic components of the Wnt signaling pathway and downregulated by the expression of dominant-negative Tcf3. A 1.4-kbp fragment of the dharma promoter region contains consensus sequences for Tcf/Lef binding sites. This promoter region recapitulated the Wnt-dependent and dorsal dharma expression pattern when it was fused to luciferase or GFP. Deletion and point mutant analyses revealed that the Tcf/Lef binding sites were required to drive this expression pattern. These data established that dharma/boz functions between the dorsal determinants-mediated Wnt signals and the formation of the Nieuwkoop center.

MeSH Terms

Animals Base Sequence Binding Sites Cytoplasm/metabolism Cytoskeletal Proteins/metabolism DNA/metabolism DNA Primers/metabolism DNA, Complementary/metabolism DNA-Binding Proteins/genetics,metabolism Down-Regulation Gene Library Genes, Dominant Genes, Reporter Green Fluorescent Proteins HMGB Proteins Homeodomain Proteins/genetics,metabolism In Situ Hybridization Luciferases/metabolism Luminescent Proteins/metabolism Lymphoid Enhancer-Binding Factor 1 Molecular Sequence Data Mutagenesis, Site-Directed Nodal Protein Plasmids/metabolism Promoter Regions, Genetic Proto-Oncogene Proteins/genetics,physiology RNA/metabolism RNA, Messenger/metabolism Signal Transduction TCF Transcription Factors Time Factors Trans-Activators Transcription Factor 7-Like 1 Protein Transcription Factors/genetics,metabolism Transforming Growth Factor beta/metabolism Up-Regulation Wnt Proteins Zebrafish Zebrafish Proteins beta Catenin

Chemicals

Cytoskeletal Proteins DNA Primers DNA, Complementary DNA-Binding Proteins HMGB Proteins Homeodomain Proteins Luminescent Proteins Lymphoid Enhancer-Binding Factor 1 Nodal Protein Proto-Oncogene Proteins RNA, Messenger TCF Transcription Factors Trans-Activators Transcription Factor 7-Like 1 Protein Transcription Factors Transforming Growth Factor beta Wnt Proteins Zebrafish Proteins beta Catenin ctnnb1 protein, zebrafish dharma protein, zebrafish wnt8b protein, zebrafish Green Fluorescent Proteins RNA DNA Luciferases

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Ryu S L

Division of Molecular Oncology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan.

Fujii R

Yamanaka Y

Shimizu T

Yabe T

Hirata T

Hibi M

Hirano T

Article Info

Journal

Developmental biology

Abbr.

Dev Biol

ISSN

0012-1606

DOI

10.1006/dbio.2000.0150

Published

2001-03-15

Pages

397-409

Language

English

Region

United States

NLM ID

0372762

Subset

External Links

PubMed source DOI: 10.1006/dbio.2000.0150 View journal details

Analysis Services

Regulation of dharma/bozozok by the Wnt pathway.

Contact

WeChat Official Account