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PMID: 11238987 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Review

Biosynthesis of polyketides in heterologous hosts.

Microbiology and molecular biology reviews : MMBR ·Vol. 65 ·No. 1 ·2001-03-00 ·Pages 106-18

Pfeifer BA, Khosla C

Abstract

Polyketide natural products show great promise as medicinal agents. Typically the products of microbial secondary biosynthesis, polyketides are synthesized by an evolutionarily related but architecturally diverse family of multifunctional enzymes called polyketide synthases. A principal limitation for fundamental biochemical studies of these modular megasynthases, as well as for their applications in biotechnology, is the challenge associated with manipulating the natural microorganism that produces a polyketide of interest. To ameliorate this limitation, over the past decade several genetically amenable microbes have been developed as heterologous hosts for polyketide biosynthesis. Here we review the state of the art as well as the difficulties associated with heterologous polyketide production. In particular, we focus on two model hosts, Streptomyces coelicolor and Escherichia coli. Future directions for this relatively new but growing technological opportunity are also discussed.

MeSH Terms
Biotechnology/methods Carrier Proteins/metabolism Escherichia coli/genetics,metabolism Fungi/genetics,metabolism Genetic Engineering/methods Multienzyme Complexes/genetics,metabolism Plants/genetics,metabolism Protein Processing, Post-Translational Recombinant Proteins/genetics,metabolism Streptomyces/genetics,metabolism Substrate Specificity
Chemicals
Carrier Proteins Multienzyme Complexes Recombinant Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pfeifer B A
Department of Chemical Engineering, Stanford University, Stanford, California 94305-5025, USA.
Khosla C
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Article Info
Journal
Microbiology and molecular biology reviews : MMBR
Abbr.
Microbiol Mol Biol Rev
ISSN
1092-2172
DOI
10.1128/MMBR.65.1.106-118.2001
Published
2001-03-00
Pages
106-18
Language
English
Region
United States
NLM ID
9706653
PMCID
PMC99020
Subset
IM
Grants
NCI NIH HHS · R01 CA066736 · United States
NCI NIH HHS · R01 CA077248 · United States
NCI NIH HHS · CA 66736 · United States
NCI NIH HHS · CA 77248 · United States
External Links
PubMed source DOI: 10.1128/MMBR.65.1.106-118.2001 PMC Full Text View journal details
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