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PMID: 11239450 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fas is required for clonal selection in germinal centers and the subsequent establishment of the memory B cell repertoire.

Immunity ·Vol. 14 ·No. 2 ·2001-02-00 ·Pages 181-92

Takahashi Y, Ohta H, Takemori T

Abstract

In T cell-dependent immune responses, high-affinity B cells are selected and differentiate into memory cells; however, the mechanism behind this process remains largely unknown. Here, we report that the selection of high-affinity B cells within germinal centers (GCs) is impaired in Fas-deficient lpr mice in the primary response, probably owing to inefficient negative selection. The memory compartment in control mice is mostly established by precursors generated from the early GCs, whereas the lpr defect expands the memory compartment by the increased recruitment of newly generated precursors from the late GCs, resulting in the accumulation of heavily mutated memory B cells at high frequency. These results suggest that Fas is required for clonal selection within GCs and the establishment of the memory B cell repertoire.

MeSH Terms

ADP-ribosyl Cyclase ADP-ribosyl Cyclase 1 Adoptive Transfer Animals Antigens, CD Antigens, Differentiation/metabolism B-Lymphocyte Subsets/cytology,immunology Base Sequence Cell Differentiation Clone Cells/cytology,immunology DNA Primers/genetics Female Genes, Immunoglobulin Germinal Center/cytology,immunology Immunoglobulin G/genetics,metabolism Immunoglobulin Heavy Chains/genetics Immunologic Memory Membrane Glycoproteins Mice Mice, Inbred C57BL Mice, Inbred MRL lpr Molecular Sequence Data Mutation NAD+ Nucleosidase/metabolism fas Receptor/genetics,metabolism

Chemicals

Antigens, CD Antigens, Differentiation DNA Primers Immunoglobulin G Immunoglobulin Heavy Chains Membrane Glycoproteins fas Receptor ADP-ribosyl Cyclase Cd38 protein, mouse NAD+ Nucleosidase ADP-ribosyl Cyclase 1

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Takahashi Y

Department of Immunology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, 162-8640, Tokyo, Japan.

Ohta H

Takemori T

Article Info

Journal

Immunity

Abbr.

Immunity

ISSN

1074-7613

DOI

10.1016/s1074-7613(01)00100-5

Published

2001-02-00

Pages

181-92

Language

English

Region

United States

NLM ID

9432918

Subset

Databases

GENBANK

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PubMed source DOI: 10.1016/s1074-7613(01)00100-5 View journal details

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Fas is required for clonal selection in germinal centers and the subsequent establishment of the memory B cell repertoire.

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