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PMID: 11252725 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Domain fusion between SNF1-related kinase subunits during plant evolution.

EMBO reports ·Vol. 2 ·No. 1 ·2001-01-00 ·Pages 55-60

Lumbreras V, Alba MM, Kleinow T, Koncz C, Pagès M

Abstract

Members of the conserved SNF1/AMP-activated protein kinase (AMPK) family regulate cellular responses to environmental and nutritional stress in eukaryotes. Yeast SNF1 and animal AMPKs form a complex with regulatory SNF4/AMPKgamma and SIP1/SIP2/GAL83/AMPKbeta subunits. The beta-subunits function as target selective adaptors that anchor the catalytic kinase and regulator SNF4/gamma-subunits to their kinase association (KIS) and association with the SNF1 complex (ASC) domains. Here we demonstrate that plant SNF1-related protein kinases (SnRKs) interact with an adaptor-regulator protein, AKINbetagamma, in which an N-terminal KIS domain characteristic of beta-subunits is fused with a C-terminal region related to the SNF4/AMPKgamma proteins. AKINbetagamma is constitutively expressed in plants, suppresses the yeast delta snf4 mutation, and shows glucose-regulated interaction with the Arabidopsis SnRK, AKIN11. Our results suggest that evolution of AKINbetagamma reflects a unique function of SNF1-related protein kinases in plant glucose and stress signalling.

MeSH Terms
AMP-Activated Protein Kinases Amino Acid Sequence Animals Arabidopsis/metabolism Bacterial Proteins/metabolism Carrier Proteins Conserved Sequence DNA, Complementary/metabolism Evolution, Molecular Gene Deletion Genetic Complementation Test Glucose/metabolism Humans Models, Biological Molecular Sequence Data Mutation Phylogeny Plants/chemistry,genetics Protein Binding Protein Kinases/genetics,metabolism Protein Serine-Threonine Kinases/chemistry,metabolism Protein Structure, Tertiary RNA, Messenger/metabolism Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Signal Transduction Stress, Physiological Temperature Time Factors Transcription Factors/genetics,metabolism Two-Hybrid System Techniques Zea mays/chemistry,genetics
Chemicals
Bacterial Proteins Carrier Proteins DNA, Complementary Kis protein, Bacteria RNA, Messenger Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors Protein Kinases SNF1-related protein kinases Protein Serine-Threonine Kinases SNF4 protein, S cerevisiae AMP-Activated Protein Kinases Glucose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lumbreras V
Departament de Genètica Molecular, Barcelona, Spain.
Alba M M
Kleinow T
Koncz C
Pagès M
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16 references, click to expand
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1093/embo-reports/kve001
Published
2001-01-00
Pages
55-60
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1083798
Subset
IM
External Links
PubMed source DOI: 10.1093/embo-reports/kve001 PMC Full Text View journal details
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