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PMID: 11257048 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hypersusceptibility of the Pseudomonas aeruginosa nfxB mutant to beta-lactams due to reduced expression of the ampC beta-lactamase.

Antimicrobial agents and chemotherapy ·Vol. 45 ·No. 4 ·2001-04-00 ·Pages 1284-6

Masuda N, Sakagawa E, Ohya S, Gotoh N, Nishino T

Abstract

The Pseudomonas aeruginosa nfxB mutant lacking mexAB-oprM showed hypersusceptibility to 9 out of 24 beta-lactams tested. This hypersusceptibility was found for the nfxB mutant lacking mexAB-oprM-mexXY (N108) but not for the nfxB mutant lacking both mexAB-oprM-mexXY and ampC. The level of the AmpC beta-lactamase induction was reduced in N108. Thus, the reduced AmpC induction must be the cause of the hypersusceptibility.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics Bacterial Proteins/genetics Carrier Proteins/genetics DNA-Binding Proteins/genetics Dose-Response Relationship, Drug Microbial Sensitivity Tests Mutation Pseudomonas aeruginosa/drug effects,enzymology Transcription Factors beta-Lactam Resistance beta-Lactamases/biosynthesis
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins DNA-Binding Proteins NfxB protein, Pseudomonas aeruginosa Transcription Factors AmpC beta-lactamases beta-Lactamases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Masuda N
Biological Research Laboratories, Sankyo Co., Ltd., Shinagawa-ku, Tokyo 140-8710, Japan.
Sakagawa E
Ohya S
Gotoh N
Nishino T
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.45.4.1284-1286.2001
Published
2001-04-00
Pages
1284-6
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC90457
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.45.4.1284-1286.2001 PMC Full Text View journal details
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