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PMID: 11259295 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Lateral organization and domain formation in a two-component lipid membrane system.

Biophysical journal ·Vol. 80 ·No. 4 ·2001-04-00 ·Pages 1819-28

Leidy C, Wolkers WF, Jørgensen K, Mouritsen OG, Crowe JH

Abstract

The thermodynamic phase behavior and lateral lipid membrane organization of unilamellar vesicles made from mixtures of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2 distearoyl-sn-glycero-3-phosphocholine (DSPC) were investigated by fluorescence resonance energy transfer (FRET) as a function of temperature and composition. This was done by incorporating a headgroup-labeled lipid donor (NBD-DPPE) and acceptor (N-Rh-DPPE) in low concentrations into the binary mixtures. Two instances of increased energy transfer efficiency were observed close to the phase lines in the DMPC/DSPC phase diagram. The increase in energy transfer efficiency was attributed to a differential preference of the probes for dynamic and fluctuating gel/fluid coexisting phases. This differential preference causes the probes to segregate (S. Pedersen, K. Jørgensen, T. R. Baekmark, and O. G. Mouritsen, 1996, Biophys. J. 71:554-560). The observed increases in energy transfer match with the boundaries of the DMPC/DSPC phase diagram, as measured by Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). We propose that the two instances of probe segregation are due to the presence of DMPC-rich and DSPC-rich domains, which form a dynamic structure of gel/fluid coexisting phases at two different temperatures. Monitoring the melting profile of each lipid component independently by FTIR shows that the domain structure is formed by DMPC-rich and DSPC-rich domains rather than by pure DMPC and DSPC domains.

MeSH Terms
Calorimetry, Differential Scanning Dimyristoylphosphatidylcholine/chemistry Membranes, Artificial Phosphatidylcholines/chemistry Phosphatidylethanolamines/chemistry Protein Structure, Tertiary Spectrometry, Fluorescence Spectroscopy, Fourier Transform Infrared Temperature Thermodynamics
Chemicals
Membranes, Artificial Phosphatidylcholines Phosphatidylethanolamines 1,2-dipalmitoyl-3-phosphatidylethanolamine Dimyristoylphosphatidylcholine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Leidy C
Biophysics and Structural Biology Graduate Group, Section of Molecular and Cellular Biology, University of California, Davis 95616, USA. [email protected]
Wolkers W F
Jørgensen K
Mouritsen O G
Crowe J H
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(01)76152-8
Email
[email protected]
Published
2001-04-00
Pages
1819-28
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1301371
Subset
IM
Grants
NHLBI NIH HHS · HL 57810-01 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(01)76152-8 PMC Full Text View journal details
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