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PMID: 11283023 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The neural recognition molecule L1 is a sialic acid-binding lectin for CD24, which induces promotion and inhibition of neurite outgrowth.

The Journal of biological chemistry ·Vol. 276 ·No. 24 ·2001-06-15 ·Pages 21656-63

Kleene R, Yang H, Kutsche M, Schachner M

Abstract

Among the recognition molecules that determine a neuron's interaction with other cells, L1 and CD24 have been suggested to cooperate with each other in neurite outgrowth and signal transduction. Here we report that binding of CD24 to L1 depends on alpha2,3-sialic acid on CD24, which determines the CD24 induced and cell type-specific promotion or inhibition of neurite outgrowth. Using knockout mutants, we could show that the CD24-induced effects on neurite outgrowth are mediated via L1, and not GPI-linked CD24, by trans-interaction of L1 with sialylated CD24. This glycoform is excluded together with L1 from raft microdomains, suggesting that molecular compartmentation in the surface membrane could play a role in signal transduction.

MeSH Terms
Amino Acid Sequence Animals Antigens, CD/chemistry,genetics,physiology Binding Sites Brain/physiology CD24 Antigen Cell Membrane/physiology Chickens Consensus Sequence Fishes Glycosylphosphatidylinositols/metabolism Humans Kinetics Leukocyte L1 Antigen Complex Membrane Glycoproteins/deficiency,genetics,physiology Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, Knockout Models, Molecular Molecular Sequence Data N-Acetylneuraminic Acid/metabolism Neural Cell Adhesion Molecules/deficiency,genetics,physiology Neurites/physiology,ultrastructure Peptide Fragments/chemistry Protein Conformation Rats Recombinant Proteins/chemistry,metabolism Sequence Alignment Sequence Homology, Amino Acid Xenopus
Chemicals
Antigens, CD CD24 Antigen CD24 protein, human Cd24a protein, mouse Glycosylphosphatidylinositols Leukocyte L1 Antigen Complex Membrane Glycoproteins Neural Cell Adhesion Molecules Peptide Fragments Recombinant Proteins N-Acetylneuraminic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kleene R
Zentrum für Molekulare Neurobiologie, Universität Hamburg, Martinistr. 52, D-20246 Hamburg, Germany.
Yang H
Kutsche M
Schachner M
Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
2001-06-15
Epub
2001-00-30
Pages
21656-63
Language
English
Region
United States
NLM ID
2985121R
Subset
IM
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