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PMID: 11283337 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA microarray analysis of cyanobacterial gene expression during acclimation to high light.

The Plant cell ·Vol. 13 ·No. 4 ·2001-04-00 ·Pages 793-806

Hihara Y, Kamei A, Kanehisa M, Kaplan A, Ikeuchi M

Abstract

DNA microarrays bearing nearly all of the genes of the unicellular cyanobacterium Synechocystis sp PCC 6803 were used to examine the temporal program of gene expression during acclimation from low to high light intensity. A complete pattern is provided of gene expression during acclimation of a photosynthetic organism to changing light intensity. More than 160 responsive genes were identified and classified into distinct sets. Genes involved in light absorption and photochemical reactions were downregulated within 15 min of exposure to high light intensity, whereas those associated with CO(2) fixation and protection from photoinhibition were upregulated. Changes in the expression of genes involved in replication, transcription, and translation, which were induced to support cellular proliferation, occurred later. Several unidentified open reading frames were induced or repressed. The possible involvement of these genes in the acclimation to high light conditions is discussed.

MeSH Terms
Adaptation, Physiological Carbon Dioxide/metabolism Cyanobacteria/genetics,metabolism DNA, Bacterial/analysis Fluorescent Dyes Gene Expression Profiling Gene Expression Regulation, Bacterial Light NADH Dehydrogenase/metabolism Oligonucleotide Array Sequence Analysis Open Reading Frames Photosynthesis Thylakoids/metabolism Transcription, Genetic
Chemicals
DNA, Bacterial Fluorescent Dyes Carbon Dioxide NADH dehydrogenase II NADH Dehydrogenase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hihara Y
Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, Urawa, Saitama 338-8570, Japan. [email protected]
Kamei A
Kanehisa M
Kaplan A
Ikeuchi M
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.13.4.793
Email
[email protected]
Published
2001-04-00
Pages
793-806
Language
English
Region
England
NLM ID
9208688
PMCID
PMC135531
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.13.4.793 PMC Full Text View journal details
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