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PMID: 11287613 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Role of NF-Y in in vivo regulation of the gamma-globin gene.

Molecular and cellular biology ·Vol. 21 ·No. 9 ·2001-05-00 ·Pages 3083-95

Duan Z, Stamatoyannopoulos G, Li Q

Abstract

The duplicated CCAAT box is required for gamma gene expression. We report here that the transcriptional factor NF-Y is recruited to the duplicated CCAAT box in vivo. A mutation of the duplicated CCAAT box that severely disrupts the NF-Y binding also reduces the accessibility level of the gamma gene promoter, affects the assembly of basal transcriptional machinery, and increases the recruitment of GATA-1 to the locus control region (LCR) and the proximal promoter and the recruitment of transcription cofactor CBP/p300 to the LCR. These findings suggest that recruitment of NF-Y to the duplicated CCAAT box plays a role in the chromatin opening of the gamma gene promoter as well as in the communication between the gamma gene promoter and the LCR.

MeSH Terms
Animals CCAAT-Binding Factor/metabolism,physiology Chromatin Gene Expression Regulation Globins/genetics HL-60 Cells Humans K562 Cells Locus Control Region Mice Promoter Regions, Genetic Transcription, Genetic Tumor Cells, Cultured
Chemicals
CCAAT-Binding Factor Chromatin Globins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Duan Z
Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington 98195, USA.
Stamatoyannopoulos G
Li Q
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.9.3083-3095.2001
Published
2001-05-00
Pages
3083-95
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC86936
Subset
IM
Grants
NIDDK NIH HHS · R56 DK045365 · United States
NHLBI NIH HHS · HL20899 · United States
NIDDK NIH HHS · R37 DK045365 · United States
NHLBI NIH HHS · R01 HL020899 · United States
NIDDK NIH HHS · R01 DK045365 · United States
NIDDK NIH HHS · DK45365 · United States
External Links
PubMed source DOI: 10.1128/MCB.21.9.3083-3095.2001 PMC Full Text View journal details
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