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PMID: 11326103 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer.

Science (New York, N.Y.) ·Vol. 292 ·No. 5517 ·2001-04-27 ·Pages 740-3

Wakayama T, Tabar V, Rodriguez I, Perry AC, Studer L, Mombaerts P

Abstract

Embryonic stem (ES) cells are fully pluripotent in that they can differentiate into all cell types, including gametes. We have derived 35 ES cell lines via nuclear transfer (ntES cell lines) from adult mouse somatic cells of inbred, hybrid, and mutant strains. ntES cells contributed to an extensive variety of cell types, including dopaminergic and serotonergic neurons in vitro and germ cells in vivo. Cloning by transfer of ntES cell nuclei could result in normal development of fertile adults. These studies demonstrate the full pluripotency of ntES cells.

MeSH Terms
Animals Blastocyst/cytology Cell Differentiation Cell Line Cell Lineage Chimera Cloning, Organism Crosses, Genetic Dopamine/metabolism Embryo Transfer Female Germ Cells/cytology Male Mice Mice, Inbred C57BL Mice, Inbred DBA Mice, Inbred ICR Mice, Nude Neurons/cytology Nuclear Transfer Techniques Serotonin/metabolism Stem Cells/cytology
Chemicals
Serotonin Dopamine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wakayama T
The Rockefeller University, New York, NY 10021, USA. [email protected]
Tabar V
Rodriguez I
Perry A C
Studer L
Mombaerts P
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.1059399
Email
[email protected]
Published
2001-04-27
Pages
740-3
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Corrections
CommentIn
PMID: 11330303
CommentIn
PMID: 11360981
External Links
PubMed source DOI: 10.1126/science.1059399 View journal details
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