Home LiteratureArticle Details
PMID: 113389 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Membrane enrichment of genetic markers close to the origin and terminus during the deoxyribonucleic acid replication cycle in Bacillus subtilis.

Journal of bacteriology ·Vol. 139 ·No. 3 ·1979-09-00 ·Pages 911-6

Beeson J, Sueoka N

Abstract

A temperature-sensitive Bacillus subtilis initiation mutant was used to achieve one cycle of synchronized deoxyribonucleic acid (DNA) replication. Markers near the origin of replication and the terminus were assayed for association with the cell membrane at intervals during the DNA replication cycle. DNA near the origin and terminus was found to be enriched in the membrane fraction throughout the DNA replication cycle. The magnitude of membrane enrichment or origin and terminus markers varied coincidentally, possibly as a consequence of incubating the cells at 45 degrees C.

MeSH Terms
Bacillus subtilis/genetics,metabolism Cell Membrane/metabolism Chromosomes, Bacterial/metabolism DNA Replication DNA, Bacterial/biosynthesis Kinetics Thymidine/metabolism Transformation, Genetic
Chemicals
DNA, Bacterial Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Beeson J
Sueoka N
References (20)
20 references, click to expand
  1. Properties of membrane-associated folded chromosomes of E. coli related to initiation and termination of DNA replication.
    Cell. 1975 Apr;4(4):337-45 PMID: 1091362
  2. Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phases.
    Proc Natl Acad Sci U S A. 1963 Apr;49:559-66 PMID: 14002700
  3. SYNCHRONOUS AND DICHOTOMOUS REPLICATIONS OF THE BACILLUS SUBTILIS CHROMOSOME DURING SPORE GERMINATION.
    Nature. 1964 Dec 12;204:1069-73 PMID: 14243385
  4. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
    J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
  5. Association of the folded chromosome with the cell envelope of Escherichia coli: nature of the membrane-associated DNA.
    J Bacteriol. 1978 Jun;134(3):1108-16 PMID: 350839
  6. Topography of chromosome membrane junction in Bacillus subtilis.
    Nat New Biol. 1973 Aug 15;244(137):204-6 PMID: 4199319
  7. Isolation of DNA-membrane complex in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4787-91 PMID: 4216023
  8. Properties of a membrane-attached form of the folded chromosome of Escherichia coli.
    J Mol Biol. 1974 Jan 5;82(1):91-105 PMID: 4594427
  9. Protein synthesis and the release of the replicated chromosome from the cell membrane.
    Nature. 1974 Sep 20;251(5472):252-4 PMID: 4607947
  10. Chromosomal sites of DNA-membrane attachment in Escherichia coli.
    J Mol Biol. 1974 Aug 5;87(2):153-68 PMID: 4610154
  11. Isolation of membrane-associated folded chromosomes from Escherichia coli: effect of protein synthesis inhibition.
    J Bacteriol. 1974 Dec;120(3):1356-63 PMID: 4612018
  12. Membrane attachment of the replication origins of a multifork (dichotomous) chromosome in Bacillus subtilis.
    J Mol Biol. 1972 Aug 21;69(2):237-48 PMID: 4627160
  13. Temperature-sensitive DNA synthesis mutants of Bacillus subtilis--appendix: theory of density transfer for symmetric chromosome replication.
    Genetics. 1973 Feb;73(2):185-214 PMID: 4633156
  14. Evidence for stable attachment of DNA to membrane at the replication origin of Escherichia coli.
    Biochem Biophys Res Commun. 1970 Oct 9;41(1):157-62 PMID: 4918012
  15. Development of competence in the Bacillus subtilis transformation system.
    J Bacteriol. 1967 Sep;94(3):562-70 PMID: 4962301
  16. Sequential replication of the Bacillus subtilis chromosome. IV. Genetic mapping by density transfer experiment.
    J Mol Biol. 1967 Jul 28;27(2):349-68 PMID: 4964542
  17. Membrane attachment of the chromosome replication origin in Bacillus subtilis.
    Cold Spring Harb Symp Quant Biol. 1968;33:695-705 PMID: 4977758
  18. Association between the chromosome and the cytoplasmic membrane in Bacillus subtilis.
    Biochem Biophys Res Commun. 1969 May 8;35(3):354-62 PMID: 4977972
  19. Envelope-associated folded chromosomes for Escherichia coli: variations under different physiological conditions.
    J Bacteriol. 1976 Aug;127(2):904-16 PMID: 783120
  20. Association of the replication terminus of the Bacillus subtilis chromosome to the cell membrane.
    J Bacteriol. 1975 Nov;124(2):1030-3 PMID: 810475
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.139.3.911-916.1979
Published
1979-09-00
Pages
911-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218038
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.139.3.911-916.1979 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2