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PMID: 11344570 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Activation of human blood monocytes by products of sensitized lymphocytes.

The Journal of clinical investigation ·Vol. 53 ·No. 2 ·1974-02-00 ·Pages 559-64

Rocklin RE, Winston CT, David JR

Abstract

Studies were carried out to determine whether products of activated human lymphocytes altered human monocyte function. Supernatants from sensitized human lymphocytes stimulated by specific antigen were cultured with monolayers of human monocytes. Such monocytes exhibited enhanced adherence to their culture vessels and increased glucose carbon-1 oxidation after 2-3 days of incubation. The substance responsible for these effects was found to elute from Sephadex G-100 gel columns in a fraction with 23,000 mol wt, the same fraction containing human migration inhibitory factor.

MeSH Terms
Cell Adhesion Chemical Fractionation Culture Media DNA/biosynthesis Dextrans Glucose/metabolism Humans Kinetics Monocytes/metabolism Oxidation-Reduction
Chemicals
Culture Media Dextrans DNA sephadex Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rocklin R E
Department of Medicine, Robert B. Brigham Hospital, Harvard Medical School, Boston, Massachusetts 02120, USA.
Winston C T
David J R
References (9)
9 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI107590
Published
1974-02-00
Pages
559-64
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC301499
Subset
IM
Grants
NIAID NIH HHS · AI-07685 · United States
NIADDK NIH HHS · AM-05577 · United States
NCRR NIH HHS · RR-05669 · United States
External Links
PubMed source DOI: 10.1172/JCI107590 PMC Full Text View journal details
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