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PMID: 11351099 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Genes that are uniquely stress regulated in salt overly sensitive (sos) mutants.

Plant physiology ·Vol. 126 ·No. 1 ·2001-05-00 ·Pages 363-75

Gong Z, Koiwa H, Cushman MA, Ray A, Bufford D, Kore-eda S, Matsumoto TK, Zhu J, Cushman JC, Bressan RA, Hasegawa PM

Abstract

Repetitive rounds of differential subtraction screening, followed by nucleotide sequence determination and northern-blot analysis, identified 84 salt-regulated (160 mM NaCl for 4 h) genes in Arabidopsis wild-type (Col-0 gl1) seedlings. Probes corresponding to these 84 genes and ACP1, RD22BP1, MYB2, STZ, and PAL were included in an analysis of salt responsive gene expression profiles in gl1 and the salt-hypersensitive mutant sos3. Six of 89 genes were expressed differentially in wild-type and sos3 seedlings; steady-state mRNA abundance of five genes (AD06C08/unknown, AD05E05/vegetative storage protein 2 [VSP2], AD05B11/S-adenosyl-L-Met:salicylic acid carboxyl methyltransferase [SAMT], AD03D05/cold regulated 6.6/inducible2 [COR6.6/KIN2], and salt tolerance zinc finger [STZ]) was induced and the abundance of one gene (AD05C10/circadian rhythm-RNA binding1 [CCR1]) was reduced in wild-type plants after salt treatment. The expression of CCR1, SAMT, COR6.6/KIN2, and STZ was higher in sos3 than in wild type, and VSP2 and AD06C08/unknown was lower in the mutant. Salt-induced expression of VSP2 in sos1 was similar to wild type, and AD06C08/unknown, CCR1, SAMT, COR6.6/KIN2, and STZ were similar to sos3. VSP2 is regulated presumably by SOS2/3 independent of SOS1, whereas the expression of the others is SOS1 dependent. AD06C08/unknown and VSP2 are postulated to be effectors of salt tolerance whereas CCR1, SAMT, COR6.6/KIN2, and STZ are determinants that must be negatively regulated during salt adaptation. The pivotal function of the SOS signal pathway to mediate ion homeostasis and salt tolerance implicates AD06C08/unknown, VSP2, SAMT, 6.6/KIN2, STZ, and CCR1 as determinates that are involved in salt adaptation.

MeSH Terms
Arabidopsis/genetics,physiology DNA, Plant Expressed Sequence Tags Gene Expression Regulation, Plant/drug effects Genes, Plant Molecular Sequence Data Mutation Open Reading Frames Sodium Chloride/pharmacology
Chemicals
DNA, Plant Sodium Chloride
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Gong Z
Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana 47907-1165, USA.
Koiwa H
Cushman M A
Ray A
Bufford D
Kore-eda S
Matsumoto T K
Zhu J
Cushman J C
Bressan R A
Hasegawa P M
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.126.1.363
Published
2001-05-00
Pages
363-75
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC102310
Subset
IM
Databases
GENBANK
AB000875, AF009228, D14712, L33677, X95573
External Links
PubMed source DOI: 10.1104/pp.126.1.363 PMC Full Text View journal details
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