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PMID: 11356187 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ubiquitin and the SCF(Grr1) ubiquitin ligase complex are involved in the signalling pathway activated by external amino acids in Saccharomyces cerevisiae.

FEBS letters ·Vol. 496 ·No. 2-3 ·2001-05-11 ·Pages 81-5

Bernard F, André B

Abstract

In response to external amino acids, the permease-like sensor Ssy1 of Saccharomyces cerevisiae activates a pathway leading to transcriptional induction of several permease genes including AGP1 and PTR2. We previously reported that AGP1 induction requires Grr1, the F-box protein part of the SCF(Grr1) ubiquitin-ligase complex. We show here that ubiquitin, other components of SCF(Grr1) and the ubiquitin-conjugating enzyme Cdc34 are essential for AGP1 and PTR2 induction. This suggests that transduction to these genes of the amino acid signal generated by Ssy1 involves an SCF(Grr1)-catalysed ubiquitination step.

MeSH Terms

Amino Acid Transport Systems Amino Acids/chemistry Anaphase-Promoting Complex-Cyclosome Carrier Proteins/biosynthesis Dose-Response Relationship, Drug F-Box Proteins Fungal Proteins/metabolism,physiology Gene Deletion Genotype Intracellular Signaling Peptides and Proteins Ligases/physiology Membrane Proteins/biosynthesis,metabolism Membrane Transport Proteins/metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Signal Transduction Time Factors Transcription, Genetic Transduction, Genetic Ubiquitin-Conjugating Enzymes Ubiquitin-Protein Ligase Complexes Ubiquitin-Protein Ligases Ubiquitins/metabolism,physiology beta-Galactosidase/metabolism

Chemicals

Amino Acid Transport Systems Amino Acids Carrier Proteins F-Box Proteins Fungal Proteins Intracellular Signaling Peptides and Proteins Membrane Proteins Membrane Transport Proteins PTR2 protein, S cerevisiae SSY1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Ubiquitins GRR1 protein, S cerevisiae CDC34 protein, S cerevisiae Ubiquitin-Conjugating Enzymes Ubiquitin-Protein Ligase Complexes Anaphase-Promoting Complex-Cyclosome Ubiquitin-Protein Ligases beta-Galactosidase Ligases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Bernard F

Laboratoire de Physiologie Cellulaire, Université Libre de Bruxelles CP 300, Institut de Biologie et de Médecine Moléculaires, rue des Pr. Jeener et Brachet 10, 6041, Gosselies, Belgium.

André B

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/s0014-5793(01)02412-7

Published

2001-05-11

Pages

81-5

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/s0014-5793(01)02412-7 View journal details

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Ubiquitin and the SCF(Grr1) ubiquitin ligase complex are involved in the signalling pathway activated by external amino acids in Saccharomyces cerevisiae.

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