Abstract
Herpes simplex virus (HSV) nucleocapsids acquire an envelope by budding through the inner nuclear membrane, but it is uncertain whether this envelope is retained during virus maturation and egress or whether mature progeny virions are derived by deenvelopment at the outer nuclear membrane followed by reenvelopment in a cytoplasmic compartment. To resolve this issue, we used immunogold electron microscopy to examine the distribution of glycoprotein D (gD) in cells infected with HSV-1 encoding a wild-type gD or a gD which is retrieved to the endoplasmic reticulum (ER). In cells infected with wild-type HSV-1, extracellular virions and virions in the perinuclear space bound approximately equal amounts of gD antibody. In cells infected with HSV-1 encoding an ER-retrieved gD, the inner and outer nuclear membranes were heavily gold labeled, as were perinuclear enveloped virions. Extracellular virions exhibited very little gold decoration (10- to 30-fold less than perinuclear virions). We conclude that the envelope of perinuclear virions must be lost during maturation and egress and that mature progeny virions must acquire an envelope from a post-ER cytoplasmic compartment. We noted also that gD appears to be excluded from the plasma membrane in cells infected with wild-type virus.
MeSH Terms
Animals
Chlorocebus aethiops
Endoplasmic Reticulum/metabolism
Herpes Simplex/virology
Herpesvirus 1, Human/metabolism
Humans
Microscopy, Electron
Nucleocapsid/metabolism
Vero Cells
Viral Envelope Proteins/genetics,metabolism
Virion/metabolism
Chemicals
Viral Envelope Proteins
glycoprotein D, Human herpesvirus 1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Skepper J N
Multi-Imaging Centre and Department of Anatomy, Department of Pathology, University of Cambridge, Cambridge, United Kingdom.
Whiteley A
Browne H
Minson A
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