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PMID: 11358866 Published · ppublish English Journal Article

Yak1p, a DYRK family kinase, translocates to the nucleus and phosphorylates yeast Pop2p in response to a glucose signal.

Genes & development ·Vol. 15 ·No. 10 ·2001-05-15 ·Pages 1217-28

Moriya H, Shimizu-Yoshida Y, Omori A, Iwashita S, Katoh M, Sakai A

Abstract

POP2 protein of Saccharomyces cerevisiae is a component of a protein complex that regulates the transcription of many genes. We found that the 97th threonine residue (Thr 97) of Pop2p was phosphorylated upon glucose limitation. The Thr 97 phosphorylation occurred within 2 min after removing glucose and was reversed within 1 min after the readdition of glucose. The effects of hexokinase mutations and glucose analogs indicate that this phosphorylation is dependent on glucose phosphorylating activity. We purified a protein kinase that phosphorylates a peptide containing Thr 97 of Pop2p and identified it as Yak1p, a DYRK family kinase. Phosphorylation of Pop2p was barely detectable in a yak1Delta strain. We found that Yak1p interacted with Bmh1p and Bmh2p only in the presence of glucose. A GFP-Yak1p fusion protein shuttled rapidly between the nucleus and the cytoplasm in response to glucose. A strain with alanine substituted for Thr 97 in Pop2p showed overgrowth in the postdiauxic transition and failed to stop the cell cycle at G(1) phase in response to glucose deprivation. Thus, Yak1p and Pop2p are part of a novel glucose-sensing system in yeast that is involved in growth control in response to glucose availability.

MeSH Terms
Carrier Proteins/metabolism Cell Division Cell Nucleus/metabolism Electrophoresis, Polyacrylamide Gel Glucose/metabolism Intracellular Signaling Peptides and Proteins Phosphorylation Protein Serine-Threonine Kinases/isolation & purification,metabolism Protein-Tyrosine Kinases/isolation & purification,metabolism Ribonucleases Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Signal Transduction Transcription Factors/metabolism
Chemicals
Carrier Proteins Intracellular Signaling Peptides and Proteins Saccharomyces cerevisiae Proteins Transcription Factors Dyrk kinase Protein-Tyrosine Kinases Protein Serine-Threonine Kinases YAK1 protein, S cerevisiae Ribonucleases POP2 protein, S cerevisiae Glucose
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Moriya H
Glucose Signaling Group, Mitsubishi Kasei Institute of Life Sciences, Tokyo 194-8511, Japan.
Shimizu-Yoshida Y
Omori A
Iwashita S
Katoh M
Sakai A
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.884001
Published
2001-05-15
Pages
1217-28
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC313799
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.884001 PMC Full Text View journal details
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