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PMID: 11368790 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the gene family encoding alternative oxidase from Candida albicans.

The Biochemical journal ·Vol. 356 ·No. Pt 2 ·2001-06-01 ·Pages 595-604

Huh WK, Kang SO

Abstract

Candida albicans possesses a cyanide-resistant respiratory pathway mediated by alternative oxidase (AOX), which seems to be encoded by a gene family with two members. Cloning and expression of AOX1a, one of the genes encoding alternative oxidase from C. albicans, has previously been reported [Huh and Kang (1999) J. Bacteriol. 181, 4098-4102]. In the present study we report the isolation of another gene coding for alternative oxidase, designated AOX1b. AOX1b contains a continuous open reading frame that encodes a polypeptide consisting of 365 amino acids. Interestingly, AOX1a and AOX1b were found to be located in tandem on one of the chromosomes of C. albicans. The presence of cyanide in the culture medium remarkably retarded the growth of the aox1a/aox1a mutants. The growth of the aox1b/aox1b mutants and the aox1a/aox1a aox1b/aox1b double mutants was almost completely inhibited in the same medium. beta-Galactosidase reporter assays indicated that, whereas AOX1a was expressed constitutively, the expression of AOX1b was dependent on growth phase and was induced by treatment with cyanide, antimycin A, H(2)O(2), menadione and paraquat. Growth of the cells in media with non-fermentable carbon sources also enhanced the expression of AOX1b. CaSLN1, which encodes a histidine kinase, seems to be involved in the regulation of AOX expression in C. albicans on the basis of the observation that the activity of cyanide-resistant respiration and the expression level of AOX in the casln1/casln1 mutants were found to be significantly low under normal conditions and slightly increased in the presence of respiratory inhibitors compared with the wild-type strain. Like AOX1a, AOX1b could also be functionally expressed in AOX-deficient Saccharomyces cerevisiae and confer cyanide-resistant respiration on the organism.

MeSH Terms
Amino Acid Sequence Base Sequence Candida albicans/drug effects,enzymology,genetics,growth & development Cyanides/pharmacology DNA Primers/genetics Gene Expression Genes, Fungal Genes, Reporter Mitochondrial Proteins Molecular Sequence Data Multigene Family Mutation Oxidoreductases/genetics Plant Proteins Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Amino Acid beta-Galactosidase/genetics
Chemicals
Cyanides DNA Primers Mitochondrial Proteins Plant Proteins Oxidoreductases alternative oxidase beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Huh W K
Laboratory of Biophysics, School of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
Kang S O
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/0264-6021:3560595
Published
2001-06-01
Pages
595-604
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1221874
Subset
IM
Databases
GENBANK
AF116872
External Links
PubMed source DOI: 10.1042/0264-6021:3560595 PMC Full Text View journal details
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