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PMID: 11387215 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter opening.

The EMBO journal ·Vol. 20 ·No. 11 ·2001-06-01 ·Pages 2823-34

Kassavetis GA, Letts GA, Geiduschek EP

Abstract

Evidence for post-recruitment functions of yeast transcription factor (TF)IIIB in initiation of transcription was first provided by the properties of TFIIIB-RNA polymerase III-promoter complexes assembled with deletion mutants of its Brf and B" subunits that are transcriptionally inactive because they fail to open the promoter. The experiments presented here show that these defects can be repaired by unpairing short (3 or 5 bp) DNA segments spanning the transcription bubble of the open promoter complex. Analysis of this suppression phenomenon indicates that TFIIIB participates in two steps of promoter opening by RNA polymerase III that are comparable to the successive steps of promoter opening by bacterial RNA polymerase holoenzyme. B" deletions between amino acids 355 and 421 interfere with the initiating step of DNA strand separation at the upstream end of the transcription bubble. Removing an N-terminal domain of Brf interferes with downstream propagation of the transcription bubble to and beyond the transcriptional start site.

MeSH Terms
Base Sequence Candida albicans/genetics DNA, Fungal/chemistry,genetics Kluyveromyces/genetics Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Heteroduplexes/chemistry,genetics Promoter Regions, Genetic Protein Subunits RNA Polymerase III/chemistry,genetics,metabolism Recombinant Proteins/chemistry,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Deletion TATA Box Templates, Genetic Transcription Factor TFIIIB Transcription Factors/chemistry,genetics,metabolism Transcription, Genetic
Chemicals
BRF1 protein, S cerevisiae DNA, Fungal Nucleic Acid Heteroduplexes Protein Subunits Recombinant Proteins Saccharomyces cerevisiae Proteins Transcription Factor TFIIIB Transcription Factors RNA Polymerase III
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kassavetis G A
Department of Biology and Center for Molecular Genetics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0634, USA. [email protected]
Letts G A
Geiduschek E P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.11.2823
Email
[email protected]
Published
2001-06-01
Pages
2823-34
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125488
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.11.2823 PMC Full Text View journal details
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